In Vitro Pdt
In Vitro Pdt - Related Videos
05:08
In Vitro Phototoxicity Assay: A PDT-based Method to Evaluate the Phototoxic Potential of a Photosensitizer in Lung Cancer Cells
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2023
Photodynamic therapy (PDT) is a non-invasive and non-surgical method for lung cancer treatment. Photosensitizers selectively accumulate in tumor tissue and lead to tumor cell death in the presence of oxygen and the proper wavelength of light. In this protocol video, PDT-based method is used to evaluate the phototoxic potential of a potential photosensitizer in lung cancer cells.
03:59
In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice
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2023
Photodynamic therapy (PDT) is a non-invasive and non-surgical method for lung cancer treatment. Photosensitizers selectively accumulate in tumor tissue and lead to tumor cell death in the presence of oxygen and the proper wavelength of light. This video describes the in vivo anti-tumor efficacy analysis using photosensitizer containing nanoparticles in a tumor-bearing mouse, to determine the phototoxic potential of the photosensitizer.
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
07:24
Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal
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2025
This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.
FAQs
The wavelength determines whether the photosensitizer receives light at a suitable activation range. When researchers adjust this condition in cultured-cell experiments, they can examine how effectively the compound is activated and how treatment effects change under different light exposures. This makes wavelength selection a central experimental variable when optimizing photodynamic therapy before clinical investigation.
Molecular oxygen enables the activated photosensitizer to generate reactive oxygen species. These chemically reactive molecules can damage cellular membranes, proteins, and genetic material, producing the biological effects measured in the model. Controlling or considering oxygen availability therefore helps researchers interpret treatment mechanisms and understand why light activation alone does not explain cellular injury.
Dose and light exposure are adjustable experimental variables rather than fixed features of every treatment. By changing them systematically, researchers can identify conditions that produce measurable therapeutic effects and compare responses across experiments. This approach helps characterize treatment intensity, examine selective cell killing, and determine which combinations merit further investigation.
A typical assessment uses cultured cells or another biological model, introduces a photosensitizer, and applies light at an appropriate wavelength in the presence of molecular oxygen. Researchers then examine the resulting therapeutic effects under defined dose and exposure conditions. Comparing these controlled treatments provides evidence about cellular damage and treatment performance.
Researchers can expose comparable biological models to different photosensitizers while examining dose, light exposure, and treatment effects. The resulting comparisons may show which compounds produce stronger or more selective cell killing under the tested conditions. Such side-by-side evidence helps identify promising candidates and clarifies how photosensitizer choice influences therapeutic performance.
These laboratory models provide a controlled setting for examining therapeutic effects and mechanisms before treatment advances to clinical investigation. Researchers can optimize photosensitizers, light conditions, dose, and exposure while assessing cellular outcomes. The findings support development of photodynamic therapy for cancer and other diseases by identifying conditions that warrant further study.