Ivis Detection System
Ivis Detection System - Related Videos
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
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Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
08:53
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
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Cited by 58 •
2009
Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
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Cited by 6 •
2013
We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
07:20
Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity
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Cited by 21 •
2012
Loss of peritoneal integrity provides a new paradigm to understand and treat chronic pelvic pain in women with mild forms of endometriosis and can be easily detected using intraoperative instillation of dye at the time of laparoscopy.
FAQs
The system can capture either bioluminescent or fluorescent signals generated by reporter molecules. These signals provide optical readouts of biological activity, while the camera records their emitted light for analysis. Selecting the appropriate signal type allows researchers to track molecular or cellular events in biological samples or living organisms over time.
A highly sensitive camera captures the emitted optical signal, and associated software maps where that signal appears and how intense it is. Researchers can therefore compare both location and signal strength across measurements. These spatial and quantitative outputs help relate optical changes to molecular or cellular activity in the imaged subject.
Reporter molecules provide the detectable bioluminescent or fluorescent signal that links an underlying biological process to an optical measurement. When their signal changes, the system can help researchers monitor associated molecular or cellular activity. This makes reporter-based imaging useful for following disease-related changes or treatment responses without relying solely on invasive sampling.
Repeated measurements in the same subject create a longitudinal view of biological change, rather than isolated observations from different subjects or time points. This approach can show how disease progression, gene expression, tumor growth, infection, or therapeutic response develops over time. It may also reduce the need for invasive sampling.
A study first uses a biological sample or living organism that produces a detectable reporter signal. The system then captures the emitted light with its sensitive camera, after which software maps signal location and quantifies intensity. Repeating this sequence over time enables researchers to compare changes within the same experimental subject.
In medicine, researchers apply IVIS imaging to preclinical investigations of disease progression, gene expression, tumor growth, infection, and therapeutic responses. Its value is greatest when the research question requires observation across multiple time points. The resulting optical measurements can support comparisons of how biological activity changes during disease or following treatment.