Muscle Patch Technique
Muscle Patch Technique - Related Videos
07:29
Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
0 Views •
Cited by 17 •
2010
Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.
09:43
Patch Clamp Electrophysiology
0 Views •
2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
0 Views •
Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
11:56
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
0 Views •
Cited by 20 •
2017
The slice patch clamp technique is an effective method for analyzing learning-induced changes in the intrinsic properties and plasticity of excitatory or inhibitory synapses.
07:19
Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique
0 Views •
2025
This protocol describes a rapid and efficient method for isolating smooth muscle cells from the rat basilar artery and recording inward rectifying potassium channel currents in these cells using the whole-cell patch clamp technique. It offers a novel approach for researchers studying the basilar artery and ion channels.
FAQs
Its main mechanical advantage is load distribution. Rather than concentrating closure forces along the edges of a weakened or open region, the muscle-based patch spans the defect and shares stress across a broader repaired area. This can make reconstruction more stable when bringing the original tissue edges together would create excessive tension, a concern in selected cardiac repairs.
Direct closure may strain fragile tissue because the repair forces remain concentrated at the original defect margins. A muscle-based graft offers an alternative surface for reconstruction, allowing the repair to bridge the affected area while reducing the need to pull vulnerable edges together tightly. This approach is most relevant when tissue weakness makes tension a major concern.
In a cardiac defect such as a ventricular septal defect, the patch is positioned across the abnormal opening to reestablish tissue continuity. By closing the communication between compartments, the repair helps maintain their intended separation. Effective positioning and integration are important because incomplete closure may leave persistent leakage through the repaired region.
The described repair involves selecting the patient’s muscle tissue or a muscle-based graft, positioning it over the weakened or open region, and securing it with sutures. The patch must cover the structural defect sufficiently to reestablish continuity and distribute stress. In cardiac surgery, this sequence supports closure or reinforcement of the targeted area.
The technique may be considered for selected congenital and acquired cardiac repairs, particularly when a weakened or open region requires reinforcement. It can be useful when direct closure would place excessive tension on fragile tissue. A ventricular septal defect is one example of a structural problem in which patch-based reconstruction may be applied.
Successful integration is reflected by greater structural stability at the repaired site and effective restoration of tissue continuity. In cardiac applications, the repair is also intended to limit persistent leakage between blood-flow compartments and reduce recurrent disruption. These outcomes indicate that the patch is functioning as a durable reinforcement rather than merely covering the defect.