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Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

DOI:
10.3791/2351-v

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07:29 min

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November 20, 2010

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,
1Vollum Institute,Oregon Health and Sciences University

Chapters

  • 00:00Title
  • 01:21Preparation of Fish for Paired Recordings
  • 03:35Paired Recordings of CaP Motor Neuron and Target Muscle Cells
  • 06:41Conclusion

Summary

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Automatic Translation

Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.

Tags

Patch Clamp RecordingMotor-neuronTarget MuscleZebrafish LarvaVertebrate Model SystemCaP Motor-neuronMorphologyLocationMicroscopic MethodsDye FillingAction Potential WaveformCell Input ResistanceLong-term RecordingsFrequency-dependence Of Synaptic TransmissionNeuromuscular JunctionQuantal SizeWhole Cell Voltage ClampUnitary EventsRelease PropertiesVesicle RecyclingSynaptic DepressionSynaptic FacilitationIn Vivo Preparation

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