Myocardial Preservation
Myocardial Preservation - Related Videos
09:45
Histological Quantification of Chronic Myocardial Infarct in Rats
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Cited by 14 •
2016
The post-mortem assessment of myocardial infarction (MI) in rodents is based on quantification of the infarct on stained heart sections. We describe an accurate method to quantify the infarct size using systematic sampling of harvested rat hearts from base to apex and image analyses of trichrome-stained histological sections.
08:15
Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
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2025
Donor heart preservation is a major determinant of success in heart transplantation. Technological advances have led to optimizing the conditions of preservation, along with a more accurate assessment of the donor heart before transplantation. We herein describe two innovative technologies currently applied for cardiac preservation: uniform cooling preservation and normothermic ex-situ heart perfusion.
07:45
Acute Myocardial Infarction in Rats
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Cited by 103 •
2011
The rat model of acute myocardial infarction (AMI) is useful to study the consequence of a MI on cardiac pathophysiological and physiological function.
08:23
LAD-Ligation: A Murine Model of Myocardial Infarction
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Cited by 81 •
2009
This video demonstrates how to use a fast and reliable model to study pathobiological and pathophysiological processes of myocardial ischemia.
07:48
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
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Cited by 37 •
2016
This protocol describes a highly reproducible model of cardiac regeneration by surgical induction of myocardial infarction in the left ventricle of postnatal day 1 mice. The method involves induction of hypothermic anesthesia and ligation of the left anterior descending coronary artery.
FAQs
Cardioplegia and hypothermia protect myocardium by addressing different demands during interrupted circulation. Cardioplegia supports creation of a motionless heart, while hypothermia lowers metabolic demand. Used together with controlled perfusion, these measures help limit depletion of cellular energy stores and preserve the conditions needed for later recovery of contractile function.
Controlled perfusion helps manage how circulation is limited and restored during a procedure. This coordination reduces unnecessary stress on heart muscle while maintaining conditions that support tissue viability. Its value becomes especially important when clinicians must balance a motionless or blood-free operative field with protection against ischemic injury.
Carefully timed restoration of circulation is central to limiting ischemia-reperfusion injury, which can occur when blood flow returns after a period of interruption. Preservation strategies therefore do not focus only on the ischemic interval. They also coordinate the return of circulation to support cellular energy stores, contractile function, and overall myocardial recovery.
Effective preservation is reflected in maintained cellular energy stores, preserved contractile function, and continued tissue viability. These outcomes connect the preservation strategy with the heart’s ability to recover after circulation resumes. They also provide a framework for comparing approaches that use cardioplegia, hypothermia, controlled perfusion, or different combinations of these measures.
During cardiac surgery, preservation combines a temporarily interrupted circulation with measures that reduce myocardial demand and protect tissue. Cardioplegia can help produce a motionless heart, hypothermia lowers metabolic requirements, and controlled perfusion helps manage circulation. Restoration is then carefully timed so the heart can resume receiving blood under protected conditions.
In heart transplantation, preservation strategies help maintain donor-heart viability while circulation is interrupted and support recovery when blood flow is restored. These methods also contribute to extending the practical window for transplantation. Consequently, myocardial preservation links operative protection with broader work on organ storage, reperfusion injury, and improved cardiac recovery.