Quadrupole Orbitrap
Quadrupole Orbitrap - Related Videos
08:10
Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
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Cited by 16 •
2017
Fizzy extraction is a new laboratory technique for analysis of volatile and semivolatile compounds. A carrier gas is dissolved in the liquid sample by applying overpressure and stirring the sample. The sample chamber is then decompressed. The analyte species are liberated to the gas phase due to effervescence.
04:19
An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
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Cited by 2 •
2023
Here, a headspace gas chromatography-tandem quadrupole mass spectrometry (HS-GC-MS/MS) method suitable for the determination of trimethylamine (TMA) in animal-derived medicines is described. The protocol includes sample pretreatment, headspace treatment, analysis conditions, methodological validation, and the determination of TMA in animal-derived medicines.
07:47
Single-Cell Mass Spectrometry Imaging Using Desorption Electrospray Ionization Coupled To Orbitrap Mass Spectrometers
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2026
This protocol describes single-cell mass spectrometry imaging of OVCAR-8 cells using desorption electrospray ionization coupled to Orbitrap mass spectrometers for high-spatial-resolution metabolomic analysis.
09:21
Mössbauer Spectroscopy
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2023
Source: Joshua Wofford, Tamara M. Powers, Department of Chemistry, Texas A&M University Mössbauer spectroscopy is a bulk characterization technique that examines the nuclear excitation of an atom by gamma rays in the solid state. The resulting Mössbauer spectrum provides information about the oxidation state, spin state, and electronic environment around the target atom, which, in combination, gives evidence about the electronic structure and ligand arrangement (geometry) of the molecule.
13:45
Introduction to Mass Spectrometry
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2023
Source: Laboratory of Dr. Khuloud Al-Jamal - King's College London Mass spectrometry is an analytical chemistry technique that enables the identification of unknown compounds within a sample, the quantification of known materials, the determination of the structure, and chemical properties of different molecules. A mass spectrometer is composed of an ionization source, an analyzer, and a detector. The process involves the ionization of chemical compounds to generate ions. When using...
FAQs
The quadrupole filters or focuses selected ions before they enter the Orbitrap analyzer. This front-end selection helps control which mass-to-charge ratio signals proceed to the trapping region, making the subsequent measurement more targeted. In complex medical samples, that staged ion handling supports precise analysis of molecules present alongside many other chemical components.
After ions enter the Orbitrap, electrostatic fields trap them and produce oscillatory motion. That motion generates an image current, an electrical signal associated with the trapped ions. The instrument applies Fourier-transform analysis to the signal, converting the measured oscillations into a mass spectrum that represents the ions according to their mass-to-charge ratios.
Accurate mass measurements and high resolving power strengthen molecular identification when samples contain many components. They help distinguish and characterize signals according to precise mass-to-charge values rather than relying only on broad or overlapping measurements. This capability is especially relevant when medical studies examine proteins, metabolites, lipids, pharmaceuticals, or biomarkers in complex samples.
The measurement proceeds through linked stages: the quadrupole first filters or focuses selected ions, those ions then enter the Orbitrap, electrostatic fields trap them, and their oscillations produce an image current. Fourier-transform processing converts that current into a mass spectrum. The resulting spectrum supports precise molecular analysis based on mass-to-charge measurements.
In medicine, this approach is useful for clinical research, drug development, pharmacokinetic studies, and investigations of disease-associated molecular changes. Its combination of sensitive analysis, accurate mass measurement, and resolving power allows researchers to examine molecular patterns in complex samples and connect measured compounds with pharmaceutical, physiological, or disease-related research questions.
The platform supports analysis of several medically important molecular categories, including proteins, metabolites, lipids, pharmaceuticals, and biomarkers. Examining these different targets allows studies to address varied questions, from characterizing molecular constituents to tracking drug-related measurements or investigating changes associated with disease. The appropriate target depends on the research objective and sample context.