Porcine Tibial Allotransplantation Model
Porcine Tibial Allotransplantation Model - Related Videos
10:31
Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model
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Cited by 5 •
2017
Currently any kind of vascularized composite allotransplantation depends on long-term-immunosuppression, difficult to support for non-life-critical indications. We present a new porcine tibial VCA model that can be used to study bone VCA and demonstrate the use of surgical angiogenesis to maintain bone viability without the need of long-term immune-modulation.
04:42
Generating a Rat Model of Tibial Neuroma Transposition
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2025
Source: Brakkee, E. M., et al. Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats. J. Vis. Exp. (2023).This video demonstrates a procedure to generate a rat tibial neuroma transposition (TNT) model. After exposing the tibial nerve in the hind leg of an anesthetized rat, transect the nerve. Next, create a subcutaneous tunnel, thread the severed nerve through the tunnel, and suture it in place. In the absence of a target for reinnervation, disorganized axonal...
09:56
A Rat Model of Tibial Cortex Transverse Transport for the Treatment of Lower Limb Ischemia
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2026
This procedure demonstrates tibial cortex transverse transport in rats, enabling visualization and analysis of revascularization during limb ischemia.
06:53
Heterotopic Hindlimb-to-Neck Transplantation in the Mouse: A Supermicrosurgical Model of Vascularized Composite Allotransplantation
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2026
We present a reproducible mouse model of heterotopic hindlimb-to-neck vascularized composite allotransplantation. This supermicrosurgical protocol, involving 0.3-0.5 mm vessel anastomoses, integrates nylon filament guidance and venous cuffing. It provides a reliable platform for translational studies on tolerance, ischemia-reperfusion injury, and immunological mechanisms in vascularized composite allotransplantation.
10:50
Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
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Cited by 38 •
2013
The tibial nerve transection model is a well-tolerated, validated, and reproducible model of skeletal muscle atrophy. The model surgical protocol is described and demonstrated in C57Black6 mice.
FAQs
Genetic distinction creates an experimental setting in which the recipient can recognize the implanted tibial tissue as biologically different. That response allows investigators to examine immune recognition and rejection mechanisms in living tissue rather than in an isolated assay. The model is therefore useful for testing whether immunosuppressive strategies alter graft acceptance and support successful bone healing.
Investigators can follow several connected outcomes: graft incorporation, revascularization, bone remodeling, and immune recognition. Incorporation indicates how the transplanted segment becomes integrated with recipient tissue, while revascularization reflects restoration of blood supply. Remodeling shows structural adaptation over time. Evaluating these outcomes together helps distinguish healing-related changes from immune-mediated graft injury.
Porcine bone provides a physiologically relevant platform because its size, structure, and healing responses resemble important aspects of human orthopedics. This similarity supports assessment of surgical reconstruction and transplantation protocols under conditions that better reflect orthopedic practice than many smaller experimental systems. Findings can consequently inform treatment development before potential clinical application.
The model permits comparison of graft responses in the presence of different immunosuppressive approaches while maintaining the biological complexity of a living recipient. Researchers can relate treatment exposure to immune recognition, rejection, graft incorporation, revascularization, and remodeling. This integrated assessment helps determine whether an approach protects the graft without overlooking structural healing outcomes important to transplantation and reconstruction.
A study begins with separate donor and recipient pigs, followed by removal of a tibial segment from the donor and implantation of that segment into the recipient. Subsequent evaluation focuses on graft incorporation, blood-vessel restoration, bone remodeling, and immune recognition. Depending on the research question, the protocol can also examine graft preservation or immunosuppressive treatment.
Researchers would choose this system when they need to study bone transplantation, rejection, graft preservation, immunosuppression, or surgical reconstruction in a large-animal setting. Its relevance is especially useful for evaluating transplantation protocols and treatments before clinical application. The model connects immune and skeletal outcomes, making it suitable when both graft survival and orthopedic healing matter.