Single Cell Plating
Single Cell Plating - Related Videos
03:38
Preparation of a Microfluidic Plate with Bacterial Cells
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2025
Source: Sutlief, A. L., et al. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (2019).This video demonstrates the preparation of a microfluidic plate for shear stress studies in bacterial cultures. After priming the channels with media, bacterial cells are introduced into the experimental channel and incubated for attachment. Residual media and culture are removed to prepare the system for subsequent shear...
09:03
Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
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2023
Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...
11:04
Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
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Cited by 4 •
2013
This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.
10:54
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Cited by 2 •
2012
Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.
09:25
Isolation and Characterization of Single Cells from Zebrafish Embryos
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Cited by 20 •
2016
This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.
FAQs
The key interpretive value is founder-to-colony linkage. When a colony develops from an isolated starting cell, its observed phenotype, lineage behavior, or treatment sensitivity can be associated with that founder rather than averaged across a mixed population. This makes Single Cell Plating useful for examining cell-to-cell variation in medically relevant samples.
Cell recovery and proliferation are central conditions for meaningful results. After cells are separated, the culture environment must support survival and expansion; otherwise, an empty site may reflect failure to grow rather than absence of a cell. Reliable interpretation therefore depends on distinguishing successful isolation from subsequent colony formation.
Low-density dispensing serves more than a logistical purpose: it increases the likelihood that culture sites contain isolated cells. That separation helps preserve the connection between an initial cell and its descendants, allowing researchers to compare colonies individually instead of treating a heterogeneous starting population as a single uniform sample.
Researchers first prepare a single-cell suspension, then dilute or dispense it at low density into separate wells or culture sites. The isolated cells are maintained under conditions that support survival and proliferation. Resulting colonies can then be examined as outputs linked to individual starting cells for downstream analysis.
Medical researchers can apply the method to tumor, immune, and stem cell populations when variation within those populations matters. Separate colonies can be evaluated for phenotype, lineage, or treatment sensitivity, helping reveal whether a response characterizes the population broadly or is associated with particular cell-derived clones.
Results from Single Cell Plating are most informative when colony-level observations remain tied to the original isolation event. Comparing colonies can expose differences in phenotype, lineage, or treatment sensitivity that a pooled culture may obscure. In medicine, this supports more resolved analysis of tumor, immune, and stem cell heterogeneity.