Von Kossa Staining
Von Kossa Staining - Related Videos
03:10
Von Kossa Staining of Aortic Vascular Smooth Muscle Cells: An In Vitro Technique for Assessment of Arterial Medial Layer Calcification
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2025
This video demonstrates a staining technique for assessing arterial medial calcification of the vascular smooth muscle cells in vitro. The von Kossa method employs a photochemical reaction to deposit metallic silver at the sites of calcification and impart a black coloration.
08:30
Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation
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Cited by 6 •
2017
In this paper, we describe a method to assess endothelial von Willebrand factor release and the subsequent platelet capture under fluid shear stress in response to inflammatory stimuli using an in vitro flow chamber system.
07:49
Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus
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Cited by 105 •
2013
Electronic von Frey measurements are an effective and improved alternative to classical von Frey filaments, as they allow rapid and precise measurement of changes in rodent mechanical withdrawal thresholds to continuously applied pressure stimuli before and after an inflammatory event.
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
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2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
FAQs
Silver ions react with anions associated with deposited mineral, particularly phosphate or carbonate salts. When the prepared section is exposed to light, the bound silver undergoes reduction to metallic silver. This deposited metallic silver creates the dark brown to black appearance that marks mineral-containing regions for microscopic assessment.
The reaction identifies mineral-associated anions rather than calcium ions themselves. Consequently, a positive signal indicates deposits containing reactive phosphate or carbonate salts, not necessarily the total amount or precise distribution of calcium. This distinction is important when interpreting calcification, bone formation, or mineral deposition in biomedical specimens.
Dark brown to black regions show where the staining reaction has identified mineral-associated salts, but they should not be interpreted as a direct calcium map. The chemical composition of deposits and the limits of the reaction affect what the image represents. Complementary stains or assays help clarify the underlying mineralization.
A typical workflow applies the silver-containing reaction to tissue sections or other prepared specimens, followed by exposure to light so bound silver becomes metallic silver. The resulting section is examined for dark brown to black deposits. The workflow therefore links chemical treatment, light-dependent signal development, and microscopic evaluation.
The technique is useful when investigators need to locate mineral deposition in biological samples. Applications include assessing ectopic calcification, examining bone or cartilage mineralization, and evaluating deposits in cultured cells or engineered tissues. These uses allow researchers to compare where mineral forms across tissues, experimental systems, or regenerative models.
In bone and cartilage research, the method reveals regions where mineral-associated salts have accumulated within tissue sections, cultured cells, or engineered tissues. Researchers can use that spatial information to assess mineral deposition as part of tissue formation studies. Because the signal is indirect for calcium, complementary assays can strengthen interpretation of the findings.