Whole Body Imaging
Whole Body Imaging - Related Videos
10:27
In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
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Cited by 13 •
2009
Bees can be conditioned in an appetitive olfactory learning paradigm (PER-conditioning). Using odors as stimuli, we established a method in which behavior is recorded while simultaneously Calcium Imaging is used to measure odor evoked activity in mushroom body neurons in vivo.
08:03
In Vivo Calcium Imaging in C. elegans Body Wall Muscles
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Cited by 3 •
2019
This method provides a way to couple optogenetics and genetically encoded calcium sensors to image baseline cytosolic calcium levels and changes in evoked calcium transients in the body wall muscles of the model organism C. elegans.
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Cited by 19 •
2016
A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.
08:31
Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
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Cited by 26 •
2015
We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.
10:32
Visualization of Flow Past a Bluff Body
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2023
Source: Ricardo Mejia-Alvarez, Hussam Hikmat Jabbar and Mahmoud N. Abdullatif, Department of Mechanical Engineering, Michigan State University, East Lansing, MI Owing to the non-linear nature of its governing laws, fluid motion induces complicated flow patterns. Understanding the nature of these patterns has been the subject of intense scrutiny for centuries. Although personal computers and supercomputers are extensively used to deduce fluid flow patterns, their capabilities are still...
FAQs
The clinical question determines whether imaging should emphasize anatomy, skeletal regions, or physiological activity. Computed tomography reconstructs X-ray measurements into anatomical images, magnetic resonance imaging derives information from magnetic fields and radiofrequency signals, and positron emission tomography maps radiotracer distribution. Choosing among these approaches affects diagnostic detail, examination time, radiation exposure, and patient safety.
Computed tomography uses ionizing radiation to acquire measurements throughout the body, then applies reconstruction algorithms to convert those measurements into interpretable images. This combination makes extensive anatomical assessment possible, but the protocol must be selected carefully because radiation exposure is part of the examination. The resulting balance influences whether CT is appropriate for a particular systemic evaluation.
Magnetic resonance imaging uses magnetic fields and radiofrequency signals to generate information about body structures without the ionizing-radiation mechanism used by CT. Positron emission tomography instead maps radiotracer distribution, providing information about physiological activity. Consequently, the two approaches can answer different clinical questions, with one emphasizing anatomical assessment and the other indicating activity across tissues.
Protocol selection must align the extent of imaging and the chosen modality with the clinical question. Important considerations include the amount of diagnostic information expected, examination time, radiation exposure, and patient safety. A coordinated approach can assess multiple organs, tissues, and skeletal regions while avoiding an unnecessarily broad or poorly matched examination.
Planning begins by identifying the medical question and the organs, tissues, or skeletal regions that require assessment. Clinicians then select an appropriate modality, define whether imaging will occur in one examination or a coordinated series, and choose a protocol that balances information, time, radiation exposure, and safety. The completed images are evaluated in relation to the clinical concern.
Clinicians may use comprehensive imaging to detect and stage disease, investigate systemic conditions, identify trauma, or assess possible metastatic spread. It can also support treatment monitoring by showing changes during care. The relevant modality depends on whether the evaluation requires primarily anatomical information, evidence of physiological activity, or assessment across several body regions.
A broad examination can show how disease or injury is distributed across multiple organs, tissues, and skeletal regions. This wider view supports staging, recognition of metastatic spread, and evaluation of systemic conditions rather than restricting interpretation to one anatomical site. When performed during treatment, repeated assessment may also help monitor disease-related changes over time.