3d Neuronal Network
3d Neuronal Network - Related Videos
02:39
Building Three-Dimensional Neuronal Networks Coupled to Micro-Electrode Arrays
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2025
This video demonstrates the development of a 3D neuronal culture coupled to a planar microelectrode array with hippocampal neurons. The isolated neurons form a 2D network on the microelectrode array's active area and adhere to glass microbeads in a multi-well plate with a membrane insert. Upon transferring these neuron-coated microbeads to the electrode, they organize into an interconnected 3D neural network.
05:45
Recording Electrophysiological Activity of the Neuronal Network in a Neuron-Astrocyte Co-culture
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2025
This video demonstrates establishing a neuron-astrocyte co-culture for recording neuronal network activity. Upon establishing the co-culture of motor neurons and astrocytes on a multi-electrode array (MEA) plate, the synchronous rhythmic electrical activity of the neuronal network was recorded extracellularly using the MEA.
02:05
Measuring the Electrophysiological Activity of Neuronal Networks Using Micro-Electrode Arrays
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2025
The video demonstrates how to use a microelectrode array (MEA) to record the electrophysiological activity of neurons derived from human induced pluripotent stem cells (hiPSCs). Electrophysiological activity from a multiwell MEA containing a neuron-astrocyte co-culture is recorded to detect action potentials from multiple neurons in the network, confirming the successful differentiation of hiPSCs into neurons.
09:47
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
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Cited by 16 •
2015
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.
09:27
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Cited by 86 •
2010
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
FAQs
The three-dimensional setting changes how neurites navigate and connect. Instead of spreading across a flat surface, neuronal processes extend through surrounding cells or biomaterials, creating spatially distributed contact points where synapses can form. This arrangement allows investigators to examine connectivity and signaling in an architecture that more closely reflects brain organization than conventional flat cultures.
Surrounding cells and biomaterials provide the physical environment through which neurites grow. Their presence gives the network a three-dimensional framework rather than leaving neuronal processes confined to a plane. This framework is important when studying how cellular organization supports synaptic connections, coordinated communication, and responses to injury or disease in a tissue-like model.
Network function depends on the interaction of electrical and chemical signaling. Neurons communicate through both forms as connections develop, and coordinated activity provides a way to assess whether the network is functioning collectively rather than as isolated cells. These signals are especially relevant for studying connectivity, neuronal plasticity, and changes produced by injury or disease.
Compared with flat cell cultures, 3D neuronal networks offer a model in which neuronal organization and neurite growth occur throughout a cellular environment. The added spatial structure can make investigations of brain organization, synaptic connectivity, and tissue responses more representative of neural tissue. Flat cultures remain a simpler comparison, while the 3D format emphasizes architecture alongside cellular behavior.
Creating a useful model requires establishing neurons within a three-dimensional cellular environment and allowing neurites to extend through surrounding cells or biomaterials. Researchers then examine whether synaptic connections form and whether electrical and chemical signals become coordinated. This workflow links structural observations, such as connectivity, with functional outcomes, such as network communication, without reducing the model to a flat layer.
Researchers can apply these networks to follow neuronal development and connectivity as the system forms. The same model can be used to investigate plasticity and to examine responses to injury or disease. Together, these uses make 3D cultures relevant for studying both normal neural organization and disrupted network behavior within a model that preserves tissue-like spatial relationships.
In neuroscience, the platform supports studies of neurological disorders and evaluation of neuroactive drugs within a more tissue-like arrangement. It also contributes to emerging tissue-engineering approaches aimed at modeling or repairing neural systems. These applications connect cellular experiments with questions about drug responses and the possibility of rebuilding organized neural function.