Adult Mouse Rpe
Adult Mouse Rpe - Related Videos
06:54
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
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Cited by 7 •
2017
The human retina is composed of functionally and molecularly distinct regions, including the fovea, macula, and peripheral retina. Here, we describe a method using punch biopsies and manual removal of tissue layers from a human eye to dissect and collect these distinct retinal regions for downstream proteomic analysis.
09:00
Olfactory Behavioral Testing in the Adult Mouse
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Cited by 68 •
2009
Fundamental, yet unique properties of the rodent olfactory system have led to its increasing study among biologists. A relatively simple assessment of its function is then also needed. Here we describe sensitive tests for the characterization of mouse olfactory sensitivity and preference.
09:18
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
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Cited by 5 •
2015
The objective of this report is to describe the protocols to derive the retinal pigment epithelium (RPE) from induced pluripotent stem (iPS) cells using different sizes of embryoid bodies.
08:42
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes
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Cited by 35 •
2016
This protocol describes a step-by-step method for the reproducible isolation and long-term culture of adult mouse cardiomyocytes with high yield, purity, and viability.
10:51
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
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Cited by 88 •
2017
Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.
FAQs
Visual-cycle recycling preserves components needed for continued light detection, while phagocytosis removes shed photoreceptor outer segments. Together, these activities reduce waste accumulation and help maintain photoreceptor function. In adult mouse RPE studies, examining either process can show how impaired epithelial support may precede or contribute to reduced neuronal survival and retinal degeneration.
The outer blood-retina barrier regulates exchange between the circulation and the neural retina while the RPE transports nutrients and waste. This makes barrier function relevant to neuronal homeostasis, not merely tissue separation. Changes in transport or barrier support can therefore provide evidence that RPE dysfunction is affecting the environment required for photoreceptor maintenance.
RPE dysfunction can connect impaired tissue maintenance with photoreceptor loss and broader retinal degeneration. Because the RPE supports neuronal survival through transport, visual-cycle recycling, and waste removal, molecular or ex vivo findings can help identify which support functions are altered. This relationship makes the tissue useful for investigating disease mechanisms rather than examining neurons in isolation.
Comparing adult mouse RPE under different biological conditions can reveal how aging or inflammation changes epithelial support of the retina. Investigators can assess consequences for retinal homeostasis, photoreceptor maintenance, and neuronal survival using ex vivo preparations or molecular analyses. These comparisons help place RPE alterations within broader processes associated with retinal degeneration.
Ex vivo preparations allow researchers to examine the tissue outside the intact animal while preserving its relevance to retinal support functions. Molecular analyses can then characterize changes associated with transport, visual-cycle recycling, phagocytosis, inflammation, or degeneration. Together, these approaches provide complementary information about tissue behavior and the molecular state underlying observed retinal effects.
Researchers can use this tissue as a foundation for evaluating genetic, pharmacological, and cell-based therapeutic strategies aimed at retinal disease. Measurements from ex vivo preparations or molecular analyses may indicate whether an intervention improves RPE-related support or limits consequences for neuronal survival. The model therefore connects treatment testing with mechanisms of retinal degeneration.