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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

DOI:
10.3791/56027-v

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10:51 min

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July 14, 2017

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, ,
1Department of Dermatology, School of Medicine,UC San Diego

Chapters

  • 00:05Title
  • 01:02Primary Mouse Neonatal Skin Keratinocyte (KC) Isolation and Culture
  • 05:06Primary Mouse Adult Tail Skin KC Isolation
  • 06:49In Vitro High Calcium-induced KC Differentiation, UVB-mediated KC Death, and KC TNFα Release Quantification
  • 07:55Results: Representative KC Imaging and Analysis
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.

Tags

Keywords: Primary Mouse KeratinocytesNeonatal Mouse SkinAdult Mouse SkinEpidermal BiologyKeratinocyte DifferentiationUVb ExposureWild Type C57 BL6 MouseSkin IsolationDispase DigestionTrypsin DigestionEpidermal IsolationCell Culture

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