Foma-1 Neurons
Foma-1 Neurons - Related Videos
12:47
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
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Cited by 8 •
2014
Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
05:45
Recording Electrophysiological Activity of the Neuronal Network in a Neuron-Astrocyte Co-culture
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2025
This video demonstrates establishing a neuron-astrocyte co-culture for recording neuronal network activity. Upon establishing the co-culture of motor neurons and astrocytes on a multi-electrode array (MEA) plate, the synchronous rhythmic electrical activity of the neuronal network was recorded extracellularly using the MEA.
03:24
Generating a Low-Density Primary Neuron Culture From Frozen Embryonic Neurons
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2025
This video demonstrates the method of culturing cryopreserved embryonic neurons, involving controlled thawing, dilution with a neurobasal medium, and seeding onto a poly-L-lysine-coated plate. Subsequent incubation supports the growth and maintenance of neuronal connections.
04:53
An Indirect Neuron-Astrocyte Co-Culture Technique for Studying Neuron-Glia Interactions
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2025
This video illustrates a method for establishing an indirect neuron-astrocyte co-culture using a compartmentalized approach. In this setup, neurons and astrocytes are spatially separated but share a conditioned medium. Neurotrophic factors released by astrocytes promote neuron differentiation and the formation of synaptic connections, suggesting neuron-glia interactions.
02:12
Direct Neuronal Reprogramming of Mouse Astrocytes into Neurons
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2025
This video showcases the direct reprogramming of astrocytes expressing transforming factors into neurons. Transformation factors trigger a cascade of molecular events facilitating astrocyte-to-neuron conversion. The process is further supported by supplying specific agents and supplements through a neuronal differentiation medium.
FAQs
Controlled conditions help limit differences between samples that could otherwise obscure treatment-related effects. By maintaining comparable cellular environments, researchers can examine changes in neuronal structure, survival, or signaling with greater consistency. This standardization is especially useful when comparing multiple experimental conditions or repeating studies designed to identify cellular responses in neuroscience.
Studies can focus on neuronal morphology, viability, and signaling. Morphology reveals structural features and treatment-associated changes, whereas viability indicates whether cells remain healthy under a tested condition. Signaling measurements address how cells respond functionally at the cellular level. Examining these readouts together provides a broader view than relying on a single observation.
Experimental treatments and environmental conditions can be evaluated by observing their effects on cellular structure, survival, or signaling. Comparing these responses under defined laboratory conditions helps distinguish changes associated with the tested factor from variation between samples. The resulting observations can clarify how a compound or environment influences neuronal cells without requiring conclusions beyond the cellular model.
Their consistent laboratory maintenance can reduce variability between samples, making results easier to compare across experimental groups and repeated studies. This is important when researchers assess subtle changes in morphology, viability, or signaling. More reproducible cellular observations can strengthen interpretation of neuronal responses and support systematic investigation of mechanisms relevant to nervous-system function.
A typical workflow begins by maintaining the cells under controlled laboratory conditions, followed by exposing them to a selected treatment or environmental condition. Researchers then examine relevant cellular outcomes, such as morphology, viability, or signaling, and compare observations across the tested conditions. This sequence links the experimental factor to measurable neuronal-cell responses.
Researchers may select this model when they need a consistent neuronal system for examining cellular responses to compounds, environmental conditions, or other experimental factors. It is particularly useful when the study emphasizes changes in structure, viability, or signaling rather than whole nervous-system behavior. The model can also support development of experimental systems for investigating neurobiological questions.
Results can show whether an experimental condition is associated with changes in neuronal morphology, cellular viability, or signaling. These findings provide evidence about responses at the cellular level and can help researchers formulate broader questions about nervous-system function. They do not by themselves describe every level of neurobiology, but they connect measurable cell behavior with larger neuroscience investigations.