Micro Electrode Recording
Micro Electrode Recording - Related Videos
09:27
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Cited by 86 •
2010
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
02:05
Measuring the Electrophysiological Activity of Neuronal Networks Using Micro-Electrode Arrays
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2025
The video demonstrates how to use a microelectrode array (MEA) to record the electrophysiological activity of neurons derived from human induced pluripotent stem cells (hiPSCs). Electrophysiological activity from a multiwell MEA containing a neuron-astrocyte co-culture is recorded to detect action potentials from multiple neurons in the network, confirming the successful differentiation of hiPSCs into neurons.
03:59
Implantation of Electrodes into a Mouse Cortex for Electrocorticographic Recordings
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2025
This video demonstrates a protocol for implanting electrocorticographic electrodes in the mouse cerebral cortex to record electrical signals in response to adeno-associated viruses that target and manipulate cofilin expression.
09:48
Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array
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Cited by 2 •
2015
We have developed an in vitro unfolded hippocampus which preserves CA1-CA3 array of neurons. Combined with the penetrating micro-electrode array, neural activity can be monitored in both the longitudinal and transverse orientations. This method provides advantages over hippocampal slice preparations as the propagation in the entire hippocampus can be recorded simultaneously.
14:19
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
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Cited by 111 •
2009
In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.
FAQs
When ion channels open, neuronal membranes depolarize, producing voltage changes that a fine electrode can detect. These changes appear as electrical activity associated with action potentials, allowing investigators to examine when neurons fire and how firing patterns vary. The resulting measurements connect cellular membrane events with activity in neural circuits.
Spontaneous firing reflects neural activity occurring without a specified experimental stimulus, whereas stimulus-evoked firing follows a sensory or other controlled intervention. Comparing these patterns helps researchers determine how neurons respond to inputs in addition to their ongoing activity. This distinction supports studies of information encoding and circuit responses.
A recording focused on an individual neuron can characterize that cell’s electrical activity and firing behavior. Signals from a small group instead provide information about activity shared across nearby neurons. This difference affects how researchers interpret the data, whether emphasizing cellular physiology or coordinated patterns within a neural circuit.
Electrode placement determines the neural activity available for measurement. Positioning near neural tissue can capture electrical signals associated with nearby activity, while placement inside tissue supports measurements closely associated with cellular events. Researchers select the position according to whether they aim to characterize individual neurons, small groups, or broader circuit activity.
A typical workflow positions a fine electrode near or inside the neural tissue of interest, detects voltage changes, and records spontaneous or stimulus-evoked firing. Researchers then examine the resulting activity to characterize neuronal physiology or responses. The same general approach can be adapted to investigate neural circuits, behavior, or experimental interventions.
Researchers apply this technique to study how neurons encode sensory information, generate behavior, and communicate within neural circuits. It also supports functional activity mapping, investigation of neurological disorders, and evaluation of responses to drugs, stimulation, or other experimental interventions. The measurements can therefore connect cellular activity with broader neural and behavioral questions.