Mounting Media Application
Mounting Media Application - Related Videos
16:30
Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
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Cited by 2 •
2007
In this report, we propose a new application of light-curing dental resins for mounting base of electrodes or microdialysis probes in chronic experiments. This material allows direct bonding to the cranium.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
09:44
Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior
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Cited by 6 •
2016
This manuscript describes an ex vivo model system comprised of organ-conditioned media derived from the lymph node, bone, lung, and brain of mice. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
13:58
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
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Cited by 26 •
2014
Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.
08:38
Microfluidic Devices for Characterizing Pore-scale Event Processes in Porous Media for Oil Recovery Applications
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Cited by 14 •
2018
The goal of this procedure is to easily and rapidly produce a microfluidic device with customizable geometry and resistance to swelling by organic fluids for oil recovery studies. A polydimethylsiloxane mold is first generated, and then used to cast the epoxy-based device. A representative displacement study is reported.
FAQs
An aqueous formulation remains in an aqueous state, whereas another formulation hardens after it is applied. This distinction identifies two physical behaviors of the mounting medium. In neuroscience imaging, the selected state must be considered alongside the tissue section and microscope objective, because the medium forms part of the specimen’s imaging interface.
Optical compatibility matters because the mounting medium lies between the biological section and the microscope objective. An appropriate interface supports clearer microscopic imaging, while poor compatibility can undermine the visual quality expected from the preparation. For brain tissue, this relationship is important when examining neurons, cellular markers, or overall neural architecture.
The medium helps maintain fluorescent or chromogenic labels around neural tissue sections, allowing those signals to remain available for microscopic examination. This support is relevant to immunofluorescence, fluorescence microscopy, and preparations using chromogenic markers. As a result, mounting becomes part of the imaging workflow for visualizing labeled neurons and other cellular features in brain sections.
Careful coverslip placement helps keep the section supported beneath a layer of mounting medium. In delicate brain tissue, that support can protect the section during examination, while the completed preparation reduces distortion and improves image clarity. These effects are especially valuable when the goal is to assess tissue organization rather than only an isolated label.
The basic workflow is to place the biological specimen in mounting medium and position a coverslip over it, creating the preparation viewed by microscopy. The medium surrounds the section, and its formulation may remain aqueous or harden after application. This sequence establishes the optical and physical conditions needed to examine the specimen.
In neuroscience, mounting media application supports histology, immunofluorescence, and fluorescence microscopy. These uses allow investigators to examine neurons, cellular markers, and neural tissue architecture in prepared brain sections. By preserving labeled structures and providing an optically compatible interface, the mounted specimen can yield clearer observations of cellular and tissue-level organization.