Mouse Nerve Surgery
Mouse Nerve Surgery - Related Videos
15:04
An Anatomical Study of Nerves at Risk During Minimally Invasive Hallux Valgus Surgery
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Cited by 9 •
2018
Minimally invasive surgical (MIS) procedures rely on anatomical references to localize structures not directly visible to the surgeon. This manuscript describes a combined method of plane-by-plane dissection and sectional anatomy of fresh-frozen specimens to locate the structures at risk during MIS procedures.
05:56
A Mouse Model of Direct Anastomosis via the Prespinal Route for Crossing Nerve Transfer Surgery
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Cited by 2 •
2021
We simulated clinical surgery to establish a protocol of direct anastomosis of bilateral brachial plexus nerves via the prespinal route in mice, contributing to the study of the neural mechanisms underlying rehabilitation upon crossing nerve transfer after central and peripheral nervous system injuries.
04:26
Testing Sensory Nerve Function in a Surgically Denervated Mouse Model
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2025
This video demonstrates testing sensory nerve function in a mouse model by surgically denervating one side and leaving the other as a control. The response to mechanical stimulation confirms the role of sensory nerves.
11:07
In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves
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Cited by 52 •
2014
Measurements of nerve conduction properties in vivo exemplify a powerful tool to characterize various animal models of neuromuscular diseases. Here, we present an easy and reliable protocol by which electrophysiological analysis on sciatic nerves of anesthetized mice can be performed.
08:01
Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
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Cited by 9 •
2016
This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.
FAQs
A standardized lesion creates a controlled form of peripheral nerve injury, allowing researchers to compare recovery across animals or treatment groups. Because the procedure can preserve surrounding tissue, changes in axon regeneration, remyelination, inflammation, and function can be linked more directly to the nerve injury and subsequent intervention rather than to unrelated tissue damage.
Reconnecting nerve ends, applying a graft, or introducing a biomaterial creates different experimental settings for examining repair. These approaches allow investigators to evaluate whether axons regenerate across an injury, whether myelin is restored, and whether inflammatory responses change during recovery. The chosen strategy therefore determines which aspects of nerve repair and therapeutic performance can be assessed.
Biological measurements reveal processes such as axon regeneration, remyelination, and inflammation, whereas sensory and motor assessments show whether those processes restore nervous-system function. Examining both types of outcome helps distinguish structural recovery from meaningful functional improvement. Tracking them over time also shows how the response develops rather than relying on a single endpoint.
These procedures provide controlled models for studying how peripheral nerves respond to injury and repair. In neuroscience, they connect cellular and tissue-level events, including axon growth, remyelination, and inflammation, with sensory or motor recovery. This relationship supports investigation of the mechanisms that determine functional outcomes after nervous-system injury.
The workflow begins with anesthesia, followed by isolation of the target nerve while surrounding tissue is preserved. Researchers then create the planned lesion or perform a repair using reconnection, a graft, or a biomaterial. Postoperative care follows the manipulation, and recovery is assessed over time through biological and functional measurements.
Fine sutures can reconnect separated nerve ends, while a graft or biomaterial can be placed at the injury site as part of the repair strategy. The specific intervention depends on the experimental question, such as examining nerve reconnection or testing a therapeutic material. Anesthesia and postoperative care support the procedure and subsequent recovery period.
Researchers use these models when they need to test nerve-repair therapies under a controlled injury condition. A treatment can be evaluated by measuring axon regeneration, remyelination, inflammatory responses, and sensory or motor recovery over time. This approach also helps determine whether an intervention affects the biological mechanisms associated with functional recovery.