Nerve Ligature
Nerve Ligature - Related Videos
08:22
Permanent Cerebral Vessel Occlusion via Double Ligature and Transection
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Cited by 14 •
2013
We describe a highly reproducible method for the permanent occlusion of a rodent major cerebral blood vessel. This technique can be accomplished with very little peripheral damage, minimal blood loss, a high rate of long-term survival, and consistent infarct volume commensurate with the human clinical population.
04:39
Induction of Neuropathic Pain in a Rat Model by Constriction of the Infraorbital Nerve
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2025
Source: Deseure, K., et al. Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain. J. Vis. Exp. (2015).This video demonstrates a method to induce neuropathic pain in a rat model by constricting the infraorbital nerve. After exposing the infraorbital nerve and tying ligatures to constrict it, the immune response at the injury site increases the excitability of neighboring neurons, which transmit pain signals to the brain.
08:23
Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats
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Cited by 154 •
2012
Due to the simplicity of surgery and the robust behavioural outcome, chronic constriction of the sciatic nerve is one of the pre-eminent animal models of neuropathic pain. Within 24 hrs following surgery, pain hypersensitivity is established and can be quantitatively measured using a von Frey aesthesiometer (mechanical test) and plantar analgesia meter (thermal test).
02:02
Inducing Orofacial Neuropathic Pain in a Rat Model via Infraorbital Nerve Ligations
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2025
This video demonstrates the induction of orofacial neuropathic pain in a rat model by ligating the infraorbital nerve. This results in nerve damage and inflammation, causing abnormal pain signaling and mimicking chronic pain conditions, which can be used to study the orofacial pain assay.
10:52
Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain
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Cited by 48 •
2015
This manuscript describes a rodent model to study trigeminal neuropathic pain. The methods described include surgical procedures to perform a chronic constriction injury of the rat’s infraorbital nerve and the post-surgical behavioral tests to measure the changes in spontaneous and evoked behavior that are indicative of persistent pain and allodynia.
FAQs
Constriction can interfere with both electrical conduction along the axon and the transport of materials within it. These disruptions alter communication between the injured nerve and connected tissues, while the local injury environment promotes inflammatory responses. Studying both effects helps researchers connect physical nerve damage with later sensory abnormalities and pain-related signaling.
Local inflammation provides a biological response to the constricted nerve and creates an opportunity to examine interactions between injured neural tissue and nearby immune cells. This context is important because nerve injury is not only an axonal event. Investigators can therefore study how inflammatory processes accompany altered nerve function and contribute to persistent sensory changes.
By disrupting normal axonal conduction, the ligature can promote abnormal electrical activity in the injured nerve. Such activity provides a mechanistic link between the physical lesion and neuropathic pain-related signaling. Researchers use this model to investigate why sensory processing becomes abnormal after peripheral nerve injury and to identify processes that may sustain pain.
The procedure creates a controlled form of peripheral nerve injury in which researchers can examine linked changes in conduction, axonal transport, inflammation, and electrical activity. This controlled setting supports focused investigation of pain signaling and sensory hypersensitivity, while also allowing study of how injured nerves interact with surrounding immune cells and later repair processes.
In the model, a suture is used to tie or constrict a peripheral nerve, with partial compression serving as the experimental injury condition. The resulting physical change is used to alter nerve function in a controlled way. Researchers then examine consequences such as disrupted signaling, inflammatory responses, abnormal electrical activity, or sensory hypersensitivity.
Researchers can examine several consequences of the induced injury, including altered pain signaling, sensory hypersensitivity, and abnormal electrical activity. The model also supports investigation of nerve regeneration and the interaction between injured nerves and surrounding immune cells. Together, these outcomes connect immediate nerve dysfunction with cellular responses and longer-term recovery or chronic pain mechanisms.
Nerve ligation models provide experimental systems for evaluating analgesic treatments against injury-associated pain responses. They also help investigators study the cellular mechanisms involved in nerve regeneration after peripheral damage. Because the same model can address pain, repair, and immune interactions, it supports comparison of treatment effects with underlying biological changes.