Rectal Suction Biopsy
Rectal Suction Biopsy - Related Videos
03:02
Immunohistochemical Staining of S-100 Protein in Human Rectal Suction Biopsy Sections
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2025
This video demonstrates the immunohistochemical staining for S-100 protein in rectal suction biopsy sections. Antigen-retrieved sections are blocked and treated with primary, followed by secondary antibodies against S-100, a marker of Hirschsprung's disease. Chromogenic substrate is added to stain the targeted cells. Samples are dehydrated and mounted before visualizing under the microscope.
05:45
Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Cited by 7 •
2019
This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.
05:32
Povidone Iodine Rectal Preparation at Time of Prostate Needle Biopsy is a Simple and Reproducible Means to Reduce Risk of Procedural Infection
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Cited by 5 •
2015
Here, we present a protocol for use at the time of transrectal ultrasound guided prostate needle biopsy (TRUS PNB) that is a simple and cost-effective means to reduce infections following the procedure.
09:39
Male Rectal Exam
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2023
Source: Joseph Donroe, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT While its usefulness in cancer screening is debated, the male rectal examination remains an important part of the physical exam. The exam is indicated in selected patients with lower urinary tract symptoms, urinary and/or fecal incontinence or retention, back pain, anorectal symptoms, abdominal complaints, trauma patients, unexplained anemia, weight loss, or bone pain. There are no absolute...
04:09
The Pocket-Creation Procedure of Endoscopic Submucosal Dissection for Large Rectal Laterally Spreading Tumors
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Cited by 1 •
2026
This study details a standardized PCM-ESD protocol for resecting large rectal LSTs, aiming to enhance dissection speed and prevent transmural perforation via submucosal tunneling.
FAQs
The main assessment concerns whether enteric ganglion cells are present, absent, or abnormally distributed within the sampled tissue. Pathologists also examine supporting nerve fibers, because their pattern can provide additional information about enteric neural organization. These observations help determine whether impaired bowel motility may reflect an abnormality in the neural structures that coordinate intestinal function.
Ganglion cells are essential components of the enteric nervous system, while supporting nerve fibers contribute to its structural organization and signaling environment. Evaluating both features gives a broader picture than assessing cellular presence alone. Their distribution can help connect clinical motility problems with altered enteric neural development or signaling, which is particularly relevant to neuroscience research.
The suction approach obtains mucosal and submucosal tissue rather than requiring a full-thickness surgical incision. This distinction makes the sampling method minimally invasive while still providing tissue for histological assessment of enteric neural structures. Full-thickness sampling examines a larger depth, whereas suction biopsy is designed to evaluate relevant neural features without the same type of surgical access.
The captured mucosal and submucosal sample undergoes histological examination. Investigators assess the tissue for ganglion cells and supporting nerve fibers, then interpret their presence, absence, or distribution in relation to suspected enteric nervous system abnormalities. The resulting findings can support diagnostic evaluation and provide information for decisions about subsequent surgical planning.
It is especially useful when Hirschsprung disease is suspected and clinicians need tissue-based evidence about enteric ganglion cells. Because the method avoids a full-thickness surgical incision, it can provide diagnostic information through a minimally invasive approach. The findings may help distinguish a neural basis for disordered bowel motility and inform planning of later treatment.
In neuroscience and pediatric gastrointestinal research, tissue findings provide a way to relate bowel dysfunction to enteric neural development and signaling. Examining ganglion cells and nerve fibers in rectal samples helps investigators study how enteric neurons contribute to coordinated bowel function. The procedure therefore connects microscopic neural organization with observable disorders of intestinal motility.