Living Brain Tissue Preparation
Living Brain Tissue Preparation - Related Videos
16:47
Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
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Cited by 50 •
2010
This protocol describes three Drosophila preparations: 1) adult brain dissection, 2) adult retina dissection and 3) developing eye disc- brain complexes dissection. Emphasis is laid on special preparation techniques and conditions for live imaging, although all preparations can be used for fixed tissue immunohistochemistry.
10:21
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition
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Cited by 2 •
2017
The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy data.
08:47
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Cited by 52 •
2010
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
03:51
Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue
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2025
The video demonstrates the preparation of a single-cell suspension of immune cells from a murine brain tissue. It involves enzymatic digestion to release cells from the brain tissue, followed by density gradient centrifugation to isolate the immune cells based on their densities to obtain the single-cell suspension.
11:55
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
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Cited by 6 •
2017
Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.
FAQs
Viability depends on maintaining suitable conditions after tissue removal. An oxygenated, nutrient-containing solution supports living neural cells during the experiment, while rapid isolation limits the time tissue spends outside those conditions. This combination allows investigators to examine neural function in a controlled preparation rather than working with a nonliving sample.
Acute slicing with a vibratome is important because sectioning can mechanically damage neural tissue. The instrument supports production of slices while limiting that damage, helping preserve functional neural circuits within the preparation. Preserved circuitry makes it possible to study synaptic transmission, neuronal excitability, and network activity under controlled experimental conditions.
Unlike experiments conducted in an intact animal, this preparation gives researchers access to living neural tissue under defined conditions and reduces the complexity of whole-animal experiments. That controlled setting helps connect cellular mechanisms with brain function. It is useful when investigators need to observe or manipulate neural activity through electrophysiology, fluorescence imaging, or pharmacological testing.
Preparation begins with rapid isolation of the relevant brain region, followed by maintenance in an oxygenated, nutrient-containing solution. Researchers then often produce acute sections with a vibratome and keep the resulting tissue viable for experiments. This sequence preserves access to neural circuits while creating a manageable sample for measurements of cellular and network function.
Two practical elements are central: a vibratome for producing acute slices and an oxygenated, nutrient-containing solution for maintaining viability. The slicing step addresses mechanical damage, whereas the solution supports the tissue after removal from the body. Together, these components create the defined conditions needed for electrophysiology, imaging, and pharmacological experiments.
Living brain tissue preparation supports several complementary readouts. Electrophysiology can examine neuronal excitability and synaptic transmission, fluorescence imaging adds an optical measurement, and pharmacological testing enables controlled assessment of neural function. Together, these approaches support studies of network activity and connect cellular measurements with brain function while reducing the complexity of whole-animal experiments.