高分辨率呼吸测量被用于确定线粒体耗氧量。这是一个简单的技术来确定线粒体呼吸链复合物'(I-IV)的呼吸率,最大线粒体电子传递系统容量,和线粒体外膜的完整性。
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| ADP | Sigma | A 4386 | 化学 |
| 抗霉素 A | Sigma | A 8674 | 化学试剂,溶于乙醇 |
| 抗坏血酸 | Merck | 1.00127 | 化学 |
| BSA | Sigma | A 6003 | 化学 |
| FCCP | Sigma | C 2920 | 化学试剂,溶于乙醇 |
| Countess 自动细胞计数仪 | Thermo Fisher Scientific | n/a | 自动细胞计数仪 |
| 细胞色素 c | Sigma | C 7752 | 化学 |
| 洋地黄皂苷 | Sigma | D 5628 | 化学试剂,溶于 |
| DMSO DMEM | Gibco | 31966021 | 培养基 |
| EGTA | fluka | 3779 | 化学 |
| FBS | Gibco | 26010-074 | 培养基成分 |
| 谷氨酸 | Sigma | G 1626 | 化学 |
| Hepes | Sigma | H 7523 | 化学 |
| KCl | 默克 | 1.04936 | 化学 |
| KH2PO< sub>4 | Merck | 1.04873 | 化学 |
| 乳糖酸钾 | Sigma | L 2398 | 化学 |
| MgCl2 | Sigma | M 9272 | 化学 |
| O2k-Core:Oxygraph-2k | Oroboros Instruments | 10000-02 | 高分辨率呼吸测定仪 |
| 寡霉素 | Sigma | O 4876 | 化学试剂,溶于乙醇 |
| 青霉素-链霉素 | Gibco | 15140122 | 化学 |
| 试剂 叠氮化钠 | Sigma | S2002 | 化学 |
| 鱼藤酮 | Sigma | R 8875 | 化学试剂,溶于乙醇 |
| 琥珀酸盐 | Sigma | S 2378 | 化学 |
| 牛磺酸 | Sigma | T 8691 | 化学 |
| TMPD | Sigma | T 3134 | 化学 |
| 胰蛋白酶 | Sigma | T 4674 | 化学 |
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