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High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
 

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

Article DOI: 10.3791/54985 08:33 min February 8th, 2017
February 8th, 2017

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Summary

High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.

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High-resolution Respirometry Mitochondrial Function Permeabilized Cells Intact Cells Mitochondrial Oxygen Consumption Syndromes Diseases Mitochondrial Dysfunction Sepsis Neurological Diseases Age-related Disorders Sensitivity Substrate On Coupled Inhibitor Titrations Experiments Biological Samples Respiration Buffer Polarographic Oxygen Sensors Cell Suspension Oxygraph Chamber Stable Oxygen Flux Signal Rotenone Injection
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