Cell Quenching
Cell Quenching - Related Videos
10:57
Quenching and Boiling
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2023
Source: Alexander S Rattner, Sanjay Adhikari, and Mahdi Nabil; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Controlled heating followed by rapid cooling is an important element of many materials processing applications. This heat-treating procedure can increase material hardness, which is important for cutting tools or surfaces in high wear environments. The rapid cooling stage is called quenching, and is often performed by immersing...
07:47
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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Cited by 7 •
2016
Quorum-quenching enzymes are anti-virulent and anti-bacterial options that can mitigate pathogenesis without risk of incurring resistance, by preventing the expression of virulence factors and genes associated with antibiotic resistance and biofilm formation. In this study, we report a method that demonstrates the efficacy of quorum-quenching enzymes in bacterial biofilm disruption.
08:07
Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
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Cited by 1 •
2025
This protocol aims to preserve cell metabolites for single-cell mass spectrometry (SCMS) by combining volatile salt solution washing, rapid liquid nitrogen quenching, freeze-drying, and −8 °C storage. It demonstrates that liquid nitrogen quenching is essential for maintaining metabolite profiles, while prolonged cold storage should be minimized to prevent metabolic alterations.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
10:55
Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
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Cited by 15 •
2015
The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.
FAQs
Rapid cooling and chilled methanol work together by lowering enzyme activity as soon as cells are collected. This reduces the opportunity for intracellular reactions to continue during handling, helping preserve relative metabolite abundances associated with the selected sampling time. The result is a sample that more closely reflects cellular biochemistry in vivo rather than changes introduced after collection.
Quenching conditions must be optimized as a set rather than treated independently. Solvent composition affects how effectively reactions are slowed, while temperature and exposure time influence both stabilization and possible metabolite leakage. Separation steps also matter because delays can permit degradation or continued activity. Testing these variables helps distinguish biological differences from artifacts created during sample preparation.
Incomplete quenching can shift measured metabolite abundances away from their original cellular values. Continued enzyme activity may consume some intermediates or generate others, whereas prolonged handling can increase degradation or leakage. Such changes can make pathway comparisons appear larger or smaller than they truly are, weakening conclusions about cellular responses and metabolic physiology.
In metabolomics, preserved samples support measurement of pathway intermediates at a chosen time point. In metabolic flux studies, those measurements help researchers examine changes in pathway behavior across experimental conditions. Using the same quenching logic across samples allows nutrient or stress responses to be compared while reducing changes caused by post-collection metabolism.
An effective workflow begins with rapid collection at a defined sampling time, immediate exposure to a chilled solvent such as methanol, and fast handling through subsequent separation steps. The sequence is designed to minimize the interval in which metabolism can continue. Consistent execution across samples improves comparability when researchers examine conditions such as nutrient availability or stress.
Cell Quenching is particularly useful when the research question concerns how metabolites change after nutrient or stress conditions. By preserving samples from each defined condition, investigators can compare cellular responses and relate altered pathway intermediates to physiology. This makes the approach valuable when timing and treatment differences must be distinguished from metabolite changes introduced during collection.
Measurements from quenched cells can provide more reliable estimates of pathway intermediates and support comparisons among experimental conditions. These data help researchers connect biochemical changes with cellular physiology, including responses associated with nutrients or stress. Interpretation remains dependent on preparation quality: if degradation, leakage, or continued reactions occur, the observed profile may not represent the cells at sampling.