Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Felista L. Tansi; Ronny R&#252;ger; Markus Rabenhold; Frank Steiniger; Alfred Fahr; Ingrid Hilger

doi:10.3791/52136

Journal

/

Bioengineering

/

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

16,146 Views

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10:55 min

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January 05, 2015

DOI:

10.3791/52136-v

DOI:

10.3791/52136-v

•

10:55 min

January 05, 2015

•

6 Views

Felista L. Tansi*¹, Ronny Rüger*², Markus Rabenhold², Frank Steiniger³, Alfred Fahr², Ingrid Hilger¹

¹Experimental Radiology, Institute of Diagnostic and Interventional Radiology I,Jena University Hospital, ²Department of Pharmaceutical Technology,Friedrich-Schiller-University Jena, ³Center for Electron Microscopy,Jena University Hospital

Summary

The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.