Facs Collection
Facs Collection - Related Videos
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
11:04
Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
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2023
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France Adoptive cell transfer is a method for introducing cells into a patient or study organism...
10:50
Isolation of Pericytes from Mouse Cortical Tissue Using FACS for Single-cell Sequencing
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Cited by 1 •
2026
To obtain mouse brain cortical pericytes meeting the demands of scRNA-seq, we present a critically optimized FACS protocol based on CD13⁺/CD31⁻ selection. Our key refinements significantly enhance cell viability and RNA integrity, enabling reliable transcriptomic profiling.
15:29
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Cited by 137 •
2010
For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.
FAQs
During FACS collection, cells travel individually through a fluid stream into a laser interrogation region. Detectors record fluorescent signals from labeled markers together with measurable physical properties. The resulting measurements allow the system to distinguish cells that match the selection criteria from those that do not, creating the basis for directing particular cells into separate collection vessels.
Physical and fluorescent measurements provide complementary information about a cell population. Fluorescent markers identify cells associated with selected characteristics, while physical properties add another measurable basis for distinguishing them. Using both types of signals helps resolve heterogeneous samples more precisely and supports enrichment of populations defined by identity, engineered status, or functional characteristics.
After laser-based detection, droplets containing cells are electrically charged according to the sorting decision. Deflection then directs those droplets into separate collection vessels. This coupling of detection, charging, and deflection converts an optical measurement into a physical separation step, allowing target cells to be recovered as enriched populations for subsequent bioengineering experiments.
Flow cytometric measurement identifies cellular properties through laser interrogation and detector signals, whereas FACS collection adds physical recovery of selected cells. The sorting stage uses electrically charged droplets and deflection to place target populations into vessels. Consequently, the output is not only information about a heterogeneous sample but also a cell population available for downstream work.
A FACS collection workflow begins as cells enter a fluid stream and pass through laser interrogation. Detectors assess their fluorescent and physical properties, and the system identifies droplets containing cells that meet the selection criteria. Electrical charging and deflection then route those droplets into separate collection vessels, producing material for later culture or analysis.
Bioengineers can use FACS collection when experiments require enriched populations from heterogeneous samples. The method supports recovery of specific cell types, engineered populations, or cells with defined functional characteristics. These populations can then contribute to culture studies, molecular analysis, tissue engineering, regenerative medicine, and the development of biomaterials or therapeutic approaches.
Recovered cell populations provide a more precise starting material for downstream experiments. Depending on the research goal, collected cells may be placed into culture, examined through molecular analysis, or incorporated into tissue-engineering and regenerative-medicine studies. Their enrichment can also support development of biomaterials and therapeutics that depend on working with defined cellular populations.
Heterogeneous samples contain cells with differing types or measurable characteristics, which can complicate interpretation and development. FACS collection helps obtain relatively pure populations before they are used in bioengineering workflows. That added control can support more precise studies of engineered cells, tissue construction, regenerative applications, and therapeutic or biomaterial development.