Malignant Peripheral Nerve Sheath Tumors
Malignant Peripheral Nerve Sheath Tumors - Related Videos
07:10
Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
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Cited by 17 •
2011
A method for reliably grafting luciferase-tagged human malignant peripheral nerve sheath tumor cells into the sciatic nerve of immunodeficient mice is described. The use of bioluminescence imaging to demonstrate proper establishment of tumor grafts and criteria for random segregation of animals into study groups are also discussed.
08:57
Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models
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2024
We have developed a methodology for assessing whether nervous system neoplasms in genetically engineered mice accurately recapitulate the pathology of their human counterparts. Here, we apply these histologic techniques, defined pathologic criteria, and culture methodologies to neurofibromas and malignant peripheral nerve sheath tumors arising in the P0-GGFβ3 mouse model.
09:37
Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
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2021
Here, we present a protocol for performing gene knockouts that are embryonic lethal in vivo in genetically engineered mouse model-derived tumors and then assessing the effect that the knockout has on tumor growth, proliferation, survival, migration, invasion, and the transcriptome in vitro and in vivo.
09:33
Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor
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Cited by 1 •
2023
We have developed a cross-species comparative oncogenomics approach utilizing genomic analyses and functional genomic screens to identify and compare therapeutic targets in tumors arising in genetically engineered mouse models and the corresponding human tumor type.
02:50
Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization
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2025
This video demonstrates the staining technique of resin-embedded, osmium tetroxide-treated rat peripheral nerve sections using toluidine blue. The stained nerve sections help visualize the fine structures of peripheral nerves.
FAQs
Genetic and epigenetic alterations can disrupt normal growth control in Schwann-lineage cells, allowing abnormal proliferation to persist. These changes also contribute to invasive behavior, including movement along nerve structures and eventual metastatic spread. Studying both alteration types helps bioengineers design cell systems that reproduce relevant tumor features rather than modeling uncontrolled growth as an isolated process.
Nerve structures provide more than a location for tumor growth: they are associated with the pattern of invasion described for these cancers. Models that include nerve-related architecture can therefore help examine how tumor cells interact with peripheral nerves and protective sheaths. This perspective may reveal behaviors that simplified, nonstructured cell cultures fail to reproduce.
The association of some tumors with neurofibromatosis type 1 or prior radiation exposure gives researchers distinct biological contexts to investigate. Bioengineered systems can be used to compare how these backgrounds relate to disrupted growth, invasion, or treatment response. Such comparisons support research into why tumors may arise through different initiating circumstances while sharing aggressive characteristics.
Three-dimensional tumor models recreate spatial relationships that are difficult to represent in flat culture systems. For MPNST research, they can help examine interactions between tumor cells, nerve-associated structures, and the surrounding microenvironment. These models also provide a platform for evaluating treatment responses under conditions intended to reflect tumor organization more closely than simpler engineered cell systems.
Biomaterials can provide engineered environments for organizing tumor and nerve-associated components, while microfluidic platforms can support controlled studies of their interactions. Together, these tools help researchers reproduce selected features of the tumor microenvironment and observe responses in defined experimental settings. Their value lies in testing specific biological questions with greater control than a general tissue model may provide.
Engineered cell systems, three-dimensional models, biomaterials, and microfluidic platforms can support examination of tumor behavior and treatment responses. By reproducing aspects of the tumor microenvironment and nerve-tumor interaction, they provide experimental settings for comparing how tumors respond to candidate interventions. The resulting evidence can contribute to development of more precise therapies, although the models represent selected features rather than the entire disease.