Tissue Engineered Vessels
Tissue Engineered Vessels - Related Videos
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
06:51
Overview of Tissue Engineering
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2023
Tissue engineering aims to create artificial tissue from biomaterials, specific cells, and growth factors. These engineered tissue constructs have far-reaching benefits, with possibilities for organ replacement and tissue repair. This video introduces the field of tissue engineering and examines the components of engineered tissue. This video also outlines some prominent methods used to create the tissue scaffold, introduce a cell population, and encourage growth and proliferation. Finally,...
16:41
Experimental Approaches to Tissue Engineering
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Cited by 2 •
2007
08:53
The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering
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Cited by 38 •
2016
We describe a microsurgical approach for the generation of an arteriovenous (AV) loop as a model for analyzing vascularization in vivo in an isolated and well-characterized environment. This model is not only useful for the investigation of angiogenesis, but is also optimally suited for engineering axially vascularized and transplantable tissues.
FAQs
Cell adhesion, proliferation, alignment, and remodeling are linked stages in developing vessel-like tissue. Biomaterial scaffolds provide a structural setting for these cellular activities, while cell-derived extracellular matrix can supply a biologically produced framework. The interaction between cells and their surrounding material influences how the construct organizes and matures under controlled culture conditions.
Controlled culture conditions support the cellular behaviors needed for tissue development, including adhesion, proliferation, alignment, and remodeling. These conditions help researchers guide the construct toward vessel-like organization before evaluating its performance. Because culture influences tissue formation, it is an important part of the bioengineering process rather than merely a storage step.
Mechanical strength, compliance, permeability, and integration with host circulation are central evaluation criteria. Strength indicates whether the construct can withstand physical demands, while compliance describes its ability to respond mechanically. Permeability provides information about transport across the vessel-like tissue, and integration assessment addresses performance in relation to the host circulation.
Alignment and remodeling indicate that cells are not simply present but are organizing and changing the construct over time. These processes contribute to the formation of vessel-like tissue and help researchers assess developmental progress. Their presence is therefore relevant when determining whether controlled culture has produced a more organized construct suitable for subsequent characterization.
Evaluation begins after the construct has developed under controlled culture conditions. Researchers examine its mechanical strength, compliance, and permeability, then consider whether it can integrate with host circulation. These measurements provide a structured basis for judging readiness for further study or potential implantation, rather than relying only on the construct’s appearance.
The principal components are living cells, biomaterial scaffolds, and cell-derived extracellular matrix. Constructs may combine cells with a scaffold or use matrix produced by the cells themselves. These options provide different material settings for adhesion, proliferation, alignment, and remodeling, allowing bioengineering studies to examine how construct composition relates to vessel-like tissue development.
Researchers may investigate these constructs as potential substitutes for damaged or diseased vasculature and as possible patient-specific grafts. They can also use them as models for vascular biology, drug responses, and regenerative medicine. Thus, their value extends beyond vascular repair: the same engineered system can support therapeutic development and controlled biological investigation.
Within bioengineering, these constructs connect material design, living cells, and tissue assessment. Researchers can study how controlled culture supports vessel-like development, then measure strength, compliance, permeability, and host-circulation integration. They can also use the constructs to investigate vascular biology and drug responses, creating experimental models that complement their potential role in regenerative medicine.