Anti-pe Microbeads
Anti-pe Microbeads - Related Videos
05:54
Microbead Implantation in the Zebrafish Embryo
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Cited by 8 •
2015
The zebrafish is an excellent model system for genetic and developmental studies. Bead implantation is a valuable tissue manipulation technique that can be used to interrogate developmental mechanisms by introducing alterations in local cellular environments. This protocol describes how to perform microbead implantation in the zebrafish embryo.
03:08
Fabrication of Amyloid-Beta-Releasing Polymer Microbeads for Modeling Alzheimer's Disease
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2025
This video demonstrates a method for fabricating polymer microbeads that encapsulate neurotoxic amyloid-beta-secreting cells. The cell-polymer suspension is processed through a microbead fabrication device, with polymer gelation achieved via calcium cross-linking, forming spherical microbeads around the cells. The polymer allows cell proliferation while allowing controlled, long-term amyloid-beta release, simulating its accumulation in Alzheimer's disease.
05:51
Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain
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Cited by 18 •
2016
Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.
08:14
Rapid Magnetic-microbead Method for Efficient Purification of Low-density Neutrophils
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Cited by 1 •
2025
Low-density neutrophils (LDN) increase significantly in several diseases. LDN are usually isolated through cell sorting. We present a practical method to obtain pure and viable LDN. After density-gradient centrifugation of peripheral blood, cells are incubated with magnetic microbeads, and then LDN are separated through magnetic columns.
09:26
A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice
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Cited by 71 •
2016
Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.
FAQs
The beads act as an additional magnetic handle for cells that already carry PE-conjugated antibodies. The antibody supplies recognition of the selected cell marker, while the bead supplies the magnetic property needed for separation. This two-part arrangement allows researchers to adapt PE-based cell labeling to magnetic enrichment or depletion without changing the antibody’s role in identifying the target population.
Retention depends on whether a cell has PE-conjugated antibodies available for Anti-PE MicroBead binding. In a magnetic field, bead-associated target cells are held back, whereas cells lacking the relevant labeling are removed during washing. The same principle supports positive selection of labeled cells or negative selection in which an unwanted population is labeled and removed.
Anti-PE MicroBeads do not independently determine which biological cells should be isolated. Specificity comes from the PE-conjugated antibody and the cell marker it recognizes; the microbead binds the antibody’s PE label. Consequently, the selected population reflects the antibody-labeling strategy, allowing isolation of defined cells from a more complex biological sample.
A typical workflow first labels the sample’s relevant cells with PE-conjugated antibodies, then adds Anti-PE MicroBeads so the beads bind the PE labels. The sample is exposed to a magnetic field, and washing removes cells that are not retained. The resulting fraction can be collected as an enriched or depleted population for subsequent analysis or study.
They are useful when a complex sample needs preliminary enrichment or removal of selected cells before flow cytometry preparation. Magnetic separation can reduce the unwanted fraction or concentrate a defined population, helping prepare material for later examination. The isolated cells may then be assessed for phenotype, function, or behavior through downstream studies.
After magnetic isolation, researchers can investigate the selected cells in studies of phenotype, function, and behavior. Enrichment or depletion provides a way to examine defined populations rather than only the original mixed sample. In biology, this connects cell-selection procedures with downstream characterization and functional comparisons among populations distinguished by antibody-recognized markers.