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Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain
DOI:

05:51 min

July 24, 2016

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Chapters

  • 00:05Title
  • 00:52Intercerebroventricular Injection of MCMV and Fluorescent Microbeads into Neonatal Mice
  • 02:41Intravenous Injection of MCMV or Fluorescent Microbeads into Neonatal Mice
  • 03:37Results: MCMV Genome Distribution in the Mouse Brain Following ICV Injection and IV Injection
  • 05:05Conclusion

Summary

Automatic Translation

Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.

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