Automated Assays

Automated assays are laboratory tests that use instruments, software, and standardized workflows to measure biological activities or molecules with limited manual handling. Typically, robotic systems dispense samples and reagents, control incubation or reaction conditions, and record signals such as fluorescence, absorbance, or luminescence for automated analysis. In biology, these assays improve throughput, consistency, and reproducibility when researchers examine many samples or experimental conditions. They support applications including enzyme activity measurements, cell-based screening, biomarker detection, and drug discovery, while reducing repetitive work and enabling quantitative comparisons across large datasets.

Automated Assays - Related Videos

Research

JoVE Journal - Biochemistry

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

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Cited by 1 •

2016

Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.

Research

JoVE Journal - Immunology and Infection
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Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

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Cited by 39 •

2012

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

Research

JoVE Journal - Behavior
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Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

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Cited by 3 •

2017

We present a behavior recording setup and protocol that enables automated analysis of the nematode, Caenorhabditis elegans' preference for soluble compounds in a population-based assay. This article describes the construction of a behavior chamber, the behavioral assay protocol, and video analysis software usage.

An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

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Cited by 28 •

2017

This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

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2025

This report describes an automated protocol for multiplex immunofluorescence (mIF) assays on an automated slide stainer. It demonstrates the high reproducibility of spatial proteomics on standard high-throughput tissue autostainers and whole-slide fluorescence imagers, which is ideal for custom mIF assays across a large number of tissue slides in translational research.

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