Bladder Detrusor Muscle
Bladder Detrusor Muscle - Related Videos
03:45
Detrusor Smooth Muscle Cell Isolation: An Enzymatic Digestion Procedure to Obtain DSM Cells from Human Urinary Bladder Specimens
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2023
In this video, we demonstrate the isolation of detrusor smooth muscle cells from human urinary bladder specimens using a two-step sequential enzymatic treatment.
06:36
A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
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Cited by 6 •
2019
The detrusor-free bladder model enables direct access to the suburothelium to study local mechanisms for regulation of biologically active mediator availability in suburothelium/lamina propria during storage and voiding of urine. The preparation closely resembles filling of an intact bladder and allows pressure-volume studies to be performed without systemic influences.
17:41
Focal Ca2+ Transient Detection in Smooth Muscle
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2009
Details methods for high-resolution Ca2+ imaging of smooth muscle within isolated organs, including: preparation of the tissue, image acquisition and data analysis.
11:46
Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
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Cited by 19 •
2014
Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition characterized in part by pelvic pain. In order to study nervous system contributions to the condition, a physiological model of urinary bladder pain is used in mice and rats.
04:15
Bladder Tumor Organoid Model: A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder
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2023
In this video, we describe the orthotopic transplantation of cultured bladder tumor organoids into the murine bladder to study the role of the organ environment on developing tumors.
FAQs
During filling, sympathetic signaling supports detrusor relaxation, while intrinsic properties of the muscle also help accommodate increasing urine volume. Stretch receptors in the bladder wall monitor changes in volume and contribute to the developing urge to urinate. Together, these mechanisms allow storage to continue while providing sensory information about bladder fullness.
When voiding begins, parasympathetic stimulation releases acetylcholine, which activates muscarinic receptors on the detrusor. This signaling increases intracellular calcium, a key step that promotes smooth muscle contraction. The receptor-mediated calcium response links neural input to force generation, allowing the bladder wall to contract and drive urine toward the urethra.
Effective urination depends on two events occurring together: the detrusor must generate pressure through contraction, and the urethral sphincter must relax to permit urine passage. If these actions are not coordinated, bladder emptying can become ineffective. Studying this relationship therefore helps explain normal micturition and how disrupted control may contribute to urinary retention.
Storage favors detrusor relaxation through sympathetic signaling and intrinsic muscle behavior, whereas voiding depends on parasympathetic activation, acetylcholine release, muscarinic receptor stimulation, and increased intracellular calcium. Stretch receptors contribute sensory information in both phases by monitoring bladder volume. The contrast between these control states allows the same organ to alternate between retaining and expelling urine.
Examining detrusor structure, neural control, receptor activity, and calcium-linked contraction helps connect normal bladder function with disease. This biological framework is relevant to overactive bladder, urinary retention, and neurogenic bladder, because each condition can involve altered storage, emptying, or communication between bladder muscle, sensory signaling, and coordinated outlet relaxation.
Research on the detrusor can clarify how smooth muscle properties, sympathetic and parasympathetic signaling, stretch detection, acetylcholine, muscarinic receptors, and intracellular calcium interact during urination. These observations provide a mechanistic view of micturition rather than treating bladder filling and emptying as isolated events. They also help identify which stage of control may be disrupted in bladder dysfunction.