Colony Screening
Colony Screening - Related Videos
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
07:47
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
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Cited by 66 •
2014
ScanLag is a high-throughput method for measuring the delay in growth, namely lag time, as well as the growth rate of colonies for thousands of cells in parallel. Screening using ScanLag enables the discrimination between long lag-time and slow growth at the level of a single variant.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
13:11
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
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Cited by 17 •
2010
Video bioinformatics is the automated processing, analysis, understanding, and data mining of biological spatio-temporal data extracted from microscopic videos. The purpose of this article is to demonstrate a method for measuring human embryonic stem cell colony growth using a video bioinformatics method.
FAQs
Selection markers enrich the population by allowing researchers to focus on colonies that meet a defined growth criterion. However, marker-based growth does not by itself establish that the intended insert is present, correctly oriented, or expressed. Researchers therefore combine selection with colony PCR, color-based reporters, or restriction analysis when characterizing candidates.
Color-based reporters provide a visible phenotype that can make candidate colonies easier to distinguish from non-target colonies. This approach links a genetic construct to an observable colony appearance, whereas colony PCR tests for the relevant DNA sequence directly. The two readouts answer different screening questions and can support subsequent selection of clones for further analysis.
Colony PCR and restriction analysis offer different ways to characterize candidate colonies. Colony PCR is suited to checking whether a target sequence is present in material from a colony, while restriction analysis helps assess DNA features such as insert presence or orientation through the resulting analysis pattern. The choice depends on the confirmation needed before further culture.
After cells have grown on solid media, researchers examine individual colonies and apply an appropriate screen. They may compare growth on replicated media, inspect a color-based reporter, or perform colony PCR or restriction analysis. Candidate colonies are then selected for further culture and analysis, reducing the work spent on non-target colonies.
Replica plating provides a way to examine corresponding colonies on additional media, supporting comparison of their growth or phenotype. It is useful when researchers need to evaluate many colonies while preserving the relationship between a candidate pattern and its source colony. Promising isolates can then undergo further characterization before downstream studies.
Within recombinant DNA experiments and bacterial transformation, screening serves as a checkpoint between colony growth and downstream work. It helps researchers avoid expanding colonies that lack the intended genetic features, while supporting strain development when candidate isolates must be compared and characterized. This links an initial colony-level observation to later culture and analysis.