Contaminant Removal
Contaminant Removal - Related Videos
07:50
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Cited by 10 •
2017
Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.
08:49
Vegetated Treatment Systems for Removing Contaminants Associated with Surface Water Toxicity in Agriculture and Urban Runoff
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Cited by 4 •
2017
This article summarizes the design attributes and the effectiveness of treatment systems that treat urban stormwater and agriculture irrigation runoff to remove pesticides and other contaminants associated with aquatic toxicity.
01:16
Contaminants and Errors
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2024
Effective sample preparation is crucial for accurate and reliable laboratory analysis. During this process, two significant sources of error can arise: concentration bias from improper sample splitting and contamination caused by methods used to reduce particle size, such as grinding or homogenization. Identifying and minimizing these potential errors is crucial to ensuring the validity of the analysis. Another key consideration is determining the appropriate number of samples required to...
01:29
Sources of Food Contamination
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2026
Contamination of food by microbial agents and natural toxins poses significant risks to public health. These hazards can be introduced at various points across the food supply chain, ranging from environmental sources to processing and storage stages. Understanding these contamination pathways is critical for developing strategies to ensure food safety.Seafood is particularly vulnerable to contamination through both environmental exposure and microbial colonization. Toxins from harmful algal...
10:20
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
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Cited by 14 •
2015
Integration of microalgal cultivation with industrial flue gas will ultimately introduce heavy metals and other inorganic compounds into the growth media. This study presents a procedure used to determine the end fate and impact of heavy metals and inorganic contaminants on the growth of Nannochloropsis salina grown in photobioreactors.
FAQs
The separation principle should match a measurable difference between the contaminant and the material being preserved. Filtration exploits particle size, centrifugation uses density, and adsorption or chromatography can separate substances through charge, solubility, or molecular affinity. Matching the mechanism to these properties improves removal while reducing the chance of losing target cells or molecules.
Removing a contaminant is useful only if the sample or biological target remains suitable for its intended analysis or use. Excessive or poorly matched treatment can compromise the material that researchers need to measure, culture, or purify. Balancing removal with preservation therefore helps reduce interference and toxicity without undermining biological interpretation or experimental reproducibility.
Selection depends on the contaminant, the target material, and the property that distinguishes them. Filtration is appropriate when particle size provides separation, whereas centrifugation is guided by density. Washing can remove unwanted substances from a material, while adsorption or chromatography is suited to separations involving charge, solubility, or molecular affinity. The sample context determines the best option.
First identify the unwanted substance and the target that must remain intact. Next determine which difference, such as size, density, charge, solubility, or affinity, can support separation. Choose a compatible method, such as filtration, centrifugation, washing, adsorption, or chromatography, and apply it with preservation of the target as the main outcome criterion.
In cell culture, removal helps limit unwanted biological or chemical substances that could interfere with growth, increase toxicity, or introduce infection risk. During biomolecule purification, it reduces materials that interfere with analysis or downstream use while retaining the desired molecules. These applications depend on choosing a separation method compatible with the cells or biomolecules being preserved.
Effective removal produces cleaner inputs for diagnostic testing, biomanufacturing, and environmental studies, as well as for culture and purification workflows. Lower contamination reduces analytical interference and supports more reliable interpretation of biological results. In practice, this can improve reproducibility because observed changes are less likely to reflect unwanted substances rather than the sample or process under investigation.