Cryostat Mounting Stage
Cryostat Mounting Stage - Related Videos
08:31
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
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Cited by 14 •
2017
This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.
06:36
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Cited by 50 •
2014
The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.
08:00
Whole-Mount In Situ Hybridization
0 Views •
2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
10:13
Method for Whole Mount Antibody Staining in Chick
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Cited by 3 •
2009
This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.
08:42
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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Cited by 13 •
2014
Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.
FAQs
The low-temperature chamber keeps the tissue sufficiently rigid for cutting, while the stage maintains support as the embedded block advances toward the microtome blade. This combination helps limit loss of structural integrity and preserves temperature-sensitive components, including lipids and materials used for enzyme histochemistry during sectioning.
Accurate mounting and alignment establish a consistent relationship between the tissue block and the blade. That positioning helps maintain section thickness, preserves the intended tissue orientation, and reduces disruption of structural features during cutting. In biological histology, these outcomes are important because microscopy and localization studies depend on sections that represent the specimen’s organization reliably.
Controlled incremental advancement moves the specimen toward the blade in measured steps, helping produce sections with reproducible thickness. Because the tissue remains supported while it advances, the stage also contributes to consistent sampling through the frozen block. This mechanical control is particularly relevant when comparing morphology or localization across multiple sections.
Preparation centers on securing the embedded frozen sample to the stage, positioning it so the tissue faces the microtome blade appropriately, and using the stage’s controlled advance during sectioning. The cryostat chamber then maintains the cold conditions needed for rigidity. Careful mounting and alignment are the main handling points that support consistent thickness and orientation across sections.
This setup is useful when rapid frozen histology is needed or when investigators must examine components that may be affected by temperature changes. The resulting sections can support microscopy, immunohistochemistry, enzyme histochemistry, and analysis of lipids or other temperature-sensitive components. These applications make the stage relevant to both diagnostic workflows and biological research.
Sections produced with this support can reveal tissue organization, disease-related changes, and the cellular localization of targets or components. In research, preserving orientation and structural integrity helps investigators relate microscopic findings to the original tissue arrangement. In diagnostic workflows, reproducible frozen sections provide a consistent basis for examining specimens rapidly with microscopy and selected staining approaches.