Culex Quinquefasciatus

Culex quinquefasciatus is a mosquito species commonly known as the southern house mosquito and an important subject in biology because it can transmit pathogens that affect humans and animals. Females acquire infectious agents while taking blood meals, then support pathogen development within their bodies before transmitting them through subsequent bites; the species typically breeds in stagnant, organically rich water. Its biology, including larval development, host-seeking behavior, and seasonal abundance, helps researchers understand mosquito ecology and disease transmission. Studying Culex quinquefasciatus supports surveillance, vector-control planning, and research on mosquito physiology, pathogen interactions, and public health.

Culex Quinquefasciatus - Related Videos

Research

JoVE Journal - Genetics
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Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

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Cited by 17 •

2020

This protocol describes microinjection procedures for Culex quinquefasciatus embryos that are optimized to work with CRISPR/Cas9 gene editing tools. This technique can efficiently generate site-specific, heritable, germline mutations that can be used for building genetic technologies in this understudied disease vector.

Research

JoVE Journal - Biology

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

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Cited by 5 •

2020

CRISPR/Cas9 is increasingly used to characterize gene function in non-model organisms. This protocol describes how to generate knock-out lines of Culex pipiens, from preparing injection mixes, to obtaining and injecting mosquito embryos, as well as how to rear, cross, and screen injected mosquitoes and their progeny for desired mutations.

Research

JoVE Journal - Biology
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AC-DC Electropenetrography for the Study of Probing and Ingestion Behaviors of Culex tarsalis Mosquitoes on Human Hands

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Cited by 1 •

2024

Procedures are described for studying the probing and ingestion behaviors of Culex tarsalis mosquitoes on human hands using AC-DC electropenetrography.

Helminth Collection and Identification from Wildlife

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Cited by 51 •

2013

Wild animals are commonly parasitized by a wide range of helminths. The four major types of helminths are “roundworms” (nematodes), “thorny-headed worms” (acanthocephalans), “flukes” (trematodes), and “tapeworms” (cestodes). Here we describe how helminths are collected from a vertebrate animal and how they are preserved and taxonomically...

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