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JoVE Journal
Genetics

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Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
 

Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

Article DOI: 10.3791/61375-v
May 24th, 2020

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Summary May 24th, 2020

This protocol describes microinjection procedures for Culex quinquefasciatus embryos that are optimized to work with CRISPR/Cas9 gene editing tools. This technique can efficiently generate site-specific, heritable, germline mutations that can be used for building genetic technologies in this understudied disease vector.

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