Disaccharides
Disaccharides - Related Videos
08:46
Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
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Cited by 3 •
2018
Here, we present protocols for the synthesis of disaccharide nucleosides by the regioselective O-glycosylation of ribonucleosides via a temporary protection of their 2',3'-diol moieties utilizing a cyclic boronic ester. This method applies to several unprotected nucleosides such as adenosine, guanosine, cytidine, uridine, 5-methyluridine, and 5-fluorouridine to give corresponding disaccharide nucleosides.
03:15
Silver Staining of Sulfated Glycosaminoglycans: A Staining Method for Visualization of Sulfated Glycosaminoglycans in Polyacrylamide Gels
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2025
In this video, we demonstrate a procedure for combined Alcian blue and silver staining of sulfated glycosaminoglycan oligosaccharide fragments. This combined staining technique significantly amplifies the bands of glycosaminoglycan polymers of different lengths in polyacrylamide gels.
14:37
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
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Cited by 7 •
2012
In this article, a high throughput method is presented for the synthesis of oligosaccharides and their attachment to the surface of polyanhydride nanoparticles for further use in targeting specific receptors on antigen presenting cells.
11:08
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
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Cited by 2 •
2018
A modular approach to the synthesis of N-glycans for attachment to an aluminum oxide-coated glass slide (ACG slide) as a glycan microarray has been developed and its use for the profiling of an HIV broadly neutralizing antibody has been demonstrated.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
FAQs
Formation depends on a condensation reaction in which two monosaccharide units become linked and water is released. The resulting glycosidic bond stores the structural connection between the sugars. During digestion, hydrolysis reverses this type of linkage by using water, producing monosaccharides that can be absorbed. This chemical reversibility connects disaccharide structure with nutritional processing.
Structural differences among sucrose, lactose, and maltose are biologically important because their sugar arrangements and glycosidic linkages are not identical. Those features influence how digestive enzymes process each compound and whether hydrolysis yields monosaccharides that can be absorbed. Identifying the linkage therefore helps explain why chemically related carbohydrates can have different biological handling in organisms.
Disaccharides contribute to biology in two connected ways: they can provide chemical energy and can function as transport forms of that energy. This makes them relevant before and after digestion: hydrolysis supplies absorbable monosaccharides, while the intact carbohydrate can represent a transported energy form. Studying both roles links molecular structure to organism-level energy use.
A biology-focused analysis can follow the molecule from structure to outcome: identify the two sugar units and their glycosidic linkage, consider condensation-based formation, examine enzymatic hydrolysis during digestion, and then relate the released monosaccharides to absorption and cellular energy metabolism. This sequence organizes interpretation without treating all disaccharides as biologically equivalent.
Lactose intolerance is a useful biological context for examining how carbohydrate processing affects nutrition. Comparing lactose with sucrose or maltose focuses attention on whether their structures and linkages are handled similarly during digestion. This comparison connects molecular differences with broader questions of nutrient processing and absorbable sugar availability, without assuming that all disaccharides have identical outcomes.
Beyond nutrition and digestion, disaccharide research supports the study of carbohydrate-based biomolecules and microbial metabolism. In these contexts, researchers can examine how sugar structures, glycosidic linkages, and hydrolysis relate to biological processing. The topic therefore connects molecular carbohydrate chemistry with wider biological systems, including organisms that use or transform carbohydrates during metabolism.