Dugesia Japonica
Dugesia Japonica - Related Videos
09:58
Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica
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Cited by 17 •
2011
An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.
10:14
Protocols for Efficient Rearing and Functional Analysis of the Endoparasitoid Wasp Asobara japonica and its Host Drosophila melanogaster
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2026
The goal of this protocol is to facilitate functional analysis of parasitoid-host interactions in Asobara japonica and Drosophila melanogaster through optimized rearing methods, a single-oviposition infection assay, and RNAi-mediated gene knockdown.
08:09
Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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Cited by 12 •
2019
Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also provided.
03:21
Genome Size and the Evolution of New Genes
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2020
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
10:24
Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
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Cited by 2 •
2017
This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.
FAQs
Neoblasts provide the proliferative cell population needed for regeneration. They divide after tissue loss, generate differentiated cell types, and contribute to rebuilding missing structures. This links stem cell activity with whole-animal recovery, allowing researchers to examine how cell production and differentiation collectively restore tissues rather than studying either process in isolation.
Regeneration requires more than producing new cells; those cells must be organized into the correct body structures. Molecular signals help maintain body organization and guide tissue patterning during recovery. Studying these signals helps explain how a regenerating animal coordinates cellular differentiation with the reconstruction of spatially arranged tissues.
Asexual fission produces new individuals when the animal separates into pieces, whereas regeneration describes the rebuilding of missing tissues after injury. The two processes are related because both depend on the organism’s ability to restore organized anatomy, but fission also represents a reproductive strategy. Comparing them helps connect regeneration with normal body organization and reproduction.
Researchers examine how the animal responds when injury removes or damages tissue. The resulting recovery provides a whole-organism context for investigating wound responses, neoblast activity, cell differentiation, and tissue patterning together. This makes it possible to connect early responses to damage with the later restoration of body organization.
Dugesia japonica combines accessible maintenance with robust regeneration and relatively simple anatomy. These features allow researchers to relate cellular events, such as stem cell proliferation and differentiation, to visible recovery of the animal. Its use as a model therefore supports studies that connect molecular signals and tissue-level processes with organism-wide outcomes.
Studies of this species address how stem cells produce differentiated tissues, how wounds initiate rebuilding, and how molecular signals preserve body organization. They also support investigation of development and asexual reproduction through fission. Together, these applications place D. japonica at the intersection of stem cell biology, developmental biology, and regenerative research.