Electron Microscopy
Electron Microscopy - Related Videos
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Cited by 12 •
2016
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 105 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
09:20
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
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Cited by 16 •
2011
This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
12:28
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
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Cited by 11 •
2017
This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.
FAQs
The principal distinction is whether the analysis emphasizes internal organization or surface architecture. Transmission electron microscopy uses ultrathin sections so electrons can pass through a specimen, supporting detailed views of internal structures such as organelles. Scanning electron microscopy instead detects electrons emitted from the surface, producing three-dimensional views that emphasize topography. Researchers choose between them according to the biological feature of interest.
Preparation is matched to the way each method forms an image. Transmission electron microscopy requires fixation, dehydration, embedding, and sectioning to create ultrathin specimens suitable for electron passage. Scanning electron microscopy may require fixation, dehydration, and coating so the specimen surface can be examined. These differing workflows determine whether the resulting image emphasizes internal structure or surface form.
Its nanometer-scale resolution allows investigators to examine structural relationships that conventional light microscopy cannot resolve. This scale is especially useful for analyzing cell ultrastructure, organelles, tissues, and pathogens, where organization depends on details smaller than those visible with ordinary microscopy. The resulting images connect cellular or material architecture with the specific biological structures being investigated.
A typical transmission workflow includes fixation, dehydration, embedding, and sectioning before imaging. The specimen is first stabilized, water is removed, and the material is embedded so ultrathin sections can be produced. Those sections allow electrons to pass through the sample and generate detailed internal images. This procedure is therefore suited to examining cellular organization and organelles.
Scanning electron microscopy preparation focuses on making the specimen surface suitable for imaging. The workflow can include fixation, dehydration, and coating, followed by detection of electrons emitted from the surface. Because the method records surface information, the resulting image emphasizes three-dimensional topography rather than internal sections. This makes it appropriate when surface architecture is the main research question.
Electron microscopy supports investigations of cell ultrastructure, organelles, tissues, pathogens, and biomaterials. Transmission images can reveal how internal components are arranged, whereas scanning images can show the form and surface organization of a specimen. By selecting the imaging mode according to the structure of interest, researchers can examine biological organization at scales unavailable to conventional microscopy.