Infrared Led Illumination

Infrared LED illumination uses light-emitting diodes to produce infrared radiation for observing, measuring, or influencing biological systems, often without visible-light interference. At a semiconductor junction, electrical current causes electron-hole recombination that releases photons, while the material’s band gap determines the emitted wavelength; tissues and specimens then absorb, scatter, reflect, or transmit this light. Infrared-sensitive cameras and detectors can capture these signals for live-animal observation, microscopy, optical sensing, and noninvasive tissue measurements. Choosing the wavelength, intensity, exposure time, and detector sensitivity is essential for obtaining clear data while limiting heating or other biological effects.

Infrared Led Illumination - Related Videos

Research

JoVE Journal - Cancer Research
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Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal

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2025

This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.

Research

JoVE Journal - Biology

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

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Cited by 1 •

2022

The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

Using Affordable LED Arrays for Photo-Stimulation of Neurons

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Cited by 7 •

2011

Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.

Phototaxis Assay of Filamentous Cyanobacteria Using Focused Light Illumination

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2025

Source: Weber, N. et. al., Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna. J. Vis. Exp. (2022)This video demonstrates a focused LED-based assay for studying phototaxis in filamentous cyanobacteria. The bacteria migrate toward the light source, forming a dense accumulation at the illuminated center, which is visualized and quantified through pixel intensity analysis of the captured image.

A Technique to Optimize Cerebral Organoid Culture Using Lateral Soft Light Illumination

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2025

This video showcases the utilization of a lateral soft light illumination technique to improve the naked-eye visualization of embryoid bodies, thereby facilitating their manipulation and transfer during cerebral organoid culture. It elucidates the setup of soft light illumination, methods for media changes and embryoid body transfer, as well as the procedure for inducing neural differentiation in embryoid bodies for cerebral organoid development.

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