Ex Vivo Vascular Reactivity
Ex Vivo Vascular Reactivity - Related Videos
07:00
The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity
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Cited by 20 •
2012
An ex vivo preparation is described for isolation of the largest gracilis muscle resistance arterioles for interrogation of both vascular responses to vasoactive stimuli and the assessment of basic structural properties via passive wall mechanics.
07:35
Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
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Cited by 12 •
2018
Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
07:46
Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
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Cited by 8 •
2015
This article describes a detailed protocol to produce a forebrain stab injury in adult mice. The stab injury induces severe reactive gliosis and glial scar formation which can be subsequently examined by standard immunohistochemistry methods.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
FAQs
The measured response can reflect activity at different vessel sites. Vasoactive agents may act on the endothelium, the cell layer lining the vessel, or directly on vascular smooth muscle. Separating these response sources helps researchers determine whether a contraction or relaxation reflects endothelial function, smooth muscle behavior, or interactions between both components.
Maintaining vessel segments under defined conditions makes responses easier to compare across treatments and samples. Because the tissue is studied outside the body, researchers can examine reactions to selected physiological signals or vasoactive compounds without the full complexity of the intact organism. This controlled setting supports clearer analysis of vascular mechanisms and drug effects.
A decrease in diameter or an increase in contractile force indicates vasoconstriction, whereas an increase in diameter or reduced force indicates vasodilation. These changes provide measurable evidence of how the vessel responds to a signal or compound. Examining both response directions helps characterize vascular function and identify effects on contraction, relaxation, or endothelial activity.
Researchers isolate vessel segments, place them in a tissue bath or organ-bath system, and maintain them under defined conditions. They then expose the preparation to physiological signals or vasoactive compounds and measure changes in diameter or force. The resulting contraction or relaxation responses are compared to characterize vascular behavior, endothelial function, or compound activity.
The approach is useful when researchers need to compare how vessels from healthy and diseased conditions respond to the same signals or compounds. Differences in contraction, relaxation, diameter, or force can indicate altered vascular mechanisms or endothelial function. Such comparisons help connect vascular behavior with disease-related changes in biology and support evaluation of potential treatments.
Potential therapeutics can be assessed by observing whether they alter vessel contraction or relaxation in isolated segments. Measuring diameter or force provides an experimental outcome for comparing drug effects under controlled conditions. In biology and cardiovascular research, these results help characterize compound activity and determine whether a treatment influences vascular muscle, endothelial responses, or overall vascular reactivity.