Fluid Collection
Fluid Collection - Related Videos
08:08
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
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Cited by 271 •
2008
Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.
02:17
Bronchoalveolar Lavage Fluid Collection from a Mouse Model Infected with Non-typeable Haemophilus influenzae
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2025
Source: Venuprasad, K., et al. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the collection and processing of bronchoalveolar lavage fluid from a euthanized mouse infected with non-typeable Haemophilus influenzae for downstream analysis.
08:50
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
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2025
In vivo, intercellular communications underpinning many central nervous system diseases, such as glioblastoma (GBM), are notoriously difficult to measure and characterize. Here, we describe procedures of cerebral open-flow microperfusion (cOFM) that can be employed to sample interstitial fluid components in a longitudinal animal model of GBM.
06:40
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
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Cited by 80 •
2018
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.
05:12
Murine Nasal Lavage Fluid Collection without Blood Contamination
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2025
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory pathogens.
FAQs
Hydrostatic pressure pushes liquid outward, whereas osmotic pressure influences movement according to differences in dissolved substances. When these forces become unbalanced, filtration can increase and liquid may accumulate in tissue spaces or cavities. Examining their interaction helps explain why fluid distribution changes in physiology and why localized collections can appear during disease.
Inflammation can alter filtration and secretion while also affecting lymphatic drainage. These changes may move more liquid into a tissue region than the normal drainage pathways can remove, producing a localized collection such as edema or an effusion. Fluid from such sites may be retrieved as wound exudate for further biological analysis.
Lymphatic drainage helps remove liquid from tissues and contributes to fluid balance. If movement into a region through filtration or secretion exceeds this removal, accumulation becomes more likely; if drainage keeps pace, excess liquid is less likely to remain localized. This relationship makes lymphatic function important when interpreting edema and other tissue fluid changes.
Collection procedures should preserve the sample’s volume, composition, and sterility. Maintaining volume supports meaningful measurement, while preserving composition helps retain the cells, proteins, pathogens, metabolites, or other biomarkers present at collection. Sterility is especially important when the sample must represent the biological state rather than contamination introduced during retrieval.
Blood, urine, cerebrospinal fluid, and wound exudate are examples of biological fluids used for analysis. Depending on the sample, investigators can examine cells, proteins, pathogens, metabolites, and other biomarkers. The resulting information can characterize biological conditions, support disease investigation, or help monitor how a condition responds to treatment.
It is useful when researchers or clinicians need a sample that reflects physiology, disease, or an experimental condition. Analysis can support diagnosis, treatment monitoring, and biological research by connecting measurable sample features with the state of an organism or system. Different collected fluids can provide complementary information through their measurable biological contents.