Fume Hood
Fume Hood - Related Videos
05:48
Fume Hoods and Laminar Flow Cabinets
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2023
Robert M. Rioux & William A. Elliott, Pennsylvania State University, University Park, PA Fume hoods and laminar flow cabinets are engineering controls that operate under similar principles. Both use a constant flow of air to prevent contamination of the laboratory environment and its inhabitants. Fume hoods prevent hazardous substances from exiting the hood workspace, whereas laminar flow cabinets prevent contaminants from entering the cabinet workspace. Fume hoods are ventilation systems...
07:07
An Introduction to Working in the Hood
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2023
Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to protect the researcher from hazardous or infectious samples. “The Hood” is a term that can include laminar flow benches, fume hoods, as well as tissue culture hoods or biosafety cabinets. All hoods operate based on the principle of laminar flow and have the same basic components, including a...
11:35
Establishment of a Chilli Thrips (Scirtothrips dorsalis Hood) Rearing System for Virulence Screening of Entomopathogenic Fungi
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Cited by 1 •
2025
A detached-leaf bioassay protocol was established for mass-rearing chilli thrips using a modified detached-leaf method in agar-containing glass containers, ensuring high survival and synchronized development. This system enables efficient screening of entomopathogenic fungi virulence against 2nd instar larvae and provides a reliable thrips supply for laboratory, greenhouse, and field evaluations.
09:50
Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
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Cited by 1 •
2018
This protocol describes the use of the chronic contractile activity model of exercise to observe stimulation-induced skeletal muscle adaptations in the rat hindlimb.
09:01
Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
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Cited by 2 •
2022
Mitochondria are key metabolic organelles that exhibit a high level of phenotypic plasticity in skeletal muscle. The import of proteins from the cytosol is a critical pathway for organelle biogenesis, essential for the expansion of the reticulum and the maintenance of mitochondrial function. Therefore, protein import serves as a barometer of cellular health.
FAQs
The hood’s inward airflow draws room air away from the researcher and toward the exhaust system. This movement helps capture airborne contaminants released during work, including vapors, gases, dust, and aerosols, before they can migrate toward the user. The same airflow also supports containment when procedures generate hazardous chemical emissions.
Sash position influences how effectively the hood contains contaminants and protects the researcher. Keeping the sash at the appropriate height helps maintain the intended inward airflow while providing a physical barrier against splashes and airborne releases. An incorrect position can reduce containment performance, making sash height a central operating condition.
Chemical compatibility helps ensure that the hood and its work area can safely accommodate the reagents used during an experiment. Waste handling is equally important because discarded chemicals can continue releasing hazardous vapors or other airborne contaminants. Considering both factors supports containment and reduces exposure risks beyond the moment of reagent preparation.
Researchers should confirm that the sash is positioned correctly, airflow is suitable for containment, and the chemicals are compatible with the hood. They should also plan appropriate waste handling before beginning work. These checks connect the hood’s physical protective function with the specific reagents and airborne hazards expected from the biological procedure.
Biology laboratories may use a fume hood when preparing fixatives, solvents, stains, and other volatile reagents. These materials can release vapors or other airborne contaminants during preparation or handling. Using the enclosure in such settings helps protect researchers while supporting routine experimental tasks that involve chemically hazardous substances.
A fume hood can help control several forms of airborne contamination, including chemical vapors, gases, dust, and aerosols. Its enclosure also helps contain splashes, while inward airflow carries released contaminants toward exhaust rather than the user. These functions make it relevant when biological work includes volatile or otherwise hazardous chemical reagents.