Thermometer Use
Thermometer Use - Related Videos
08:44
Fabrication and Testing of Photonic Thermometers
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Cited by 1 •
2018
We describe the process of fabrication and testing of photonic thermometers.
01:22
Thermometers and Temperature Scales
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2023
Any physical property that depends consistently and reproducibly on temperature can be used as the basis of a thermometer. For example, volume increases with temperature for most substances. This property is the basis for the common alcohol thermometer and the original mercury thermometers. Other properties used to measure temperature include electrical resistance, color, and the emission of infrared radiation. As many physical properties depend on temperature, the variety of thermometers is...
01:22
Gas Thermometers and the Kelvin Scale
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2023
The definition of temperature in terms of molecular motion suggests that there should be a lowest possible temperature, where the average kinetic energy of molecules is zero (or the minimum allowed by quantum mechanics). Experiments confirm the existence of such a temperature, called absolute zero. An absolute temperature scale is one whose zero point is absolute zero. Such scales are convenient in science because several physical quantities, such as the volume of an ideal gas, are directly...
04:34
Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation
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Cited by 44 •
2018
Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results.
06:11
Boiling Points - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Boiling point determination of acetone and ethanolExpand The temperature at which a pure organic substance changes from the liquid phase to the gas phase is known as the boiling point. A liquid's boiling point can be determined using the capillary method, where an inverted capillary is placed in the liquid of interest and the liquid is heated. As the temperature increases, the air in the capillary...
FAQs
Thermometers rely on a measurable property that changes predictably with heat. In liquid devices, expansion changes the liquid level; electrical instruments detect changes in resistance; infrared systems respond to emitted infrared energy. These mechanisms allow temperature to be converted into a readable value, but the suitable choice depends on whether the measurement involves direct contact or sensing without disturbing the biological system.
Placement determines whether the reading represents the material or organism of interest rather than a nearby environment. A thermometer positioned incorrectly may measure surrounding air, a container surface, or another location with a different temperature. Correct positioning is especially important when monitoring cultures, reactions, or organisms because inaccurate readings can lead to incorrect conclusions about the conditions affecting biological activity.
Temperature affects metabolism, molecular interactions, growth, and survival, so even a measurement that appears close to the intended condition can influence an experiment’s outcome. Changes in temperature may alter cell-culture behavior or enzyme-reaction performance, while unsuitable conditions can affect organismal physiology. Monitoring temperature therefore helps researchers connect observed biological changes with controlled experimental conditions.
Infrared sensing can be considered when obtaining a measurement without disturbing the system is important. Because infrared instruments detect emitted energy rather than relying on direct contact, they may help preserve the condition of the biological material being observed. The measurement approach should still match the experimental question and provide a reading that represents the relevant biological location or surface.
First, select a thermometer whose sensing method suits the system and the intended measurement. Position it so that it represents the relevant biological material or environment without unnecessarily disturbing the system. Read and record the value consistently, then continue monitoring when conditions may change. This workflow supports controlled experiments and makes temperature-related differences easier to interpret.
In cell cultures, temperature readings help verify that conditions remain suitable for cellular growth and survival. During enzyme experiments, measurements help establish whether the reaction environment remains consistent while molecular interactions occur. Regular, correctly positioned readings provide evidence that temperature was controlled, allowing researchers to interpret changes in culture behavior or reaction results with greater confidence.
Physiological processes respond to physical conditions, including temperature. By measuring temperature during a study, researchers can relate observations about an organism to the conditions present during the experiment rather than assuming those conditions remained constant. This is useful for distinguishing biological responses associated with temperature from changes caused by other experimental factors and for documenting conditions relevant to reproducibility.