Gamma Ray Irradiation
Gamma Ray Irradiation - Related Videos
02:17
Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs
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2025
This video demonstrates the measurement of trans-epithelial electrical resistance in irradiated Caco-2 cells, both in the presence and absence of PBMC cells. This method enables the study of the synergistic effects of radiation and immune cells on the epithelial cell barrier.
09:31
X-ray Diffraction
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2023
Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA X-ray diffraction (XRD) is a technique used in materials science for determining the atomic and molecular structure of a material. This is done by irradiating a sample of the material with incident X-rays and then measuring the intensities and scattering angles of the X-rays that are scattered by the material. The intensity of the scattered X-rays are plotted as a function of the...
10:09
Planarian Immobilization, Partial Irradiation, and Tissue Transplantation
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Cited by 20 •
2012
An effective method for grafting tissue of defined and consistent size between planaria is described. Also included is a description of how the immobilization technique used for transplantation can be adapted, in conjunction with lead shields, for the partial irradiation of live animals.
06:51
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
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Cited by 14 •
2021
This document describes a new dosimetry protocol for cell irradiations using low energy X-ray equipment. Measurements are performed in conditions simulating real cell irradiation conditions as much as possible.
11:40
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
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Cited by 7 •
2018
We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.
FAQs
Gamma ray irradiation harms cells through two linked routes: photons can ionize cellular molecules directly, and irradiation can generate reactive oxygen species that produce additional chemical damage. These reactive compounds can affect DNA, proteins, and membranes. Considering both routes helps explain why one exposure may cause broad cellular injury rather than a single isolated molecular change.
The biological outcome depends on more than whether exposure occurred. Dose, exposure time, and cellular sensitivity determine the extent of injury, so changing one variable can alter cell damage or survival. Controlled comparisons allow researchers to relate measured effects to radiation level and exposure duration while accounting for differences in how biological materials respond.
DNA damage connects irradiation with both cellular injury and broader radiation responses. By examining dose-dependent effects alongside cell survival, researchers can investigate how cells repair damage and why some biological systems tolerate exposure better than others. This framework links molecular damage with observable biological outcomes without treating all cells as equally sensitive.
Researchers control exposure conditions, vary dose or exposure time, and compare biological outcomes across conditions. Measurements of dose-dependent injury, microbial reduction, or cell survival provide a basis for interpreting the experiment. This approach supports systematic analysis of radiation responses and helps connect the amount of irradiation with the degree of biological change observed.
Laboratory supplies can be treated to reduce microbial contamination before biological work. The method damages microbial DNA, proteins, and membranes, thereby lowering the number of viable contaminants associated with treated materials. This application gives researchers a controlled way to prepare supplies for experiments and helps limit contamination that could interfere with biological observations.
At controlled exposures, irradiation can induce mutations that researchers study in biological systems, including crops. This application extends the method beyond sterilization: instead of seeking microbial reduction, investigators examine altered biological characteristics or responses. It provides material for biological research and crop improvement while allowing outcomes to be considered alongside dose-dependent radiation effects.