Gelatinized Plates
Gelatinized Plates - Related Videos
07:33
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
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Cited by 5 •
2015
This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.
09:11
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
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Cited by 10 •
2016
We present a facile method to fabricate a biodegradable gelatin-based drug release platform that is magneto-thermally responsive. This was achieved by incorporating superparamagnetic iron oxide nanoparticles and poly(N-isopropylacrylamide-co-acrylamide) within a spherical gelatin micro-network crosslinked by genipin, in conjunction with an alternating magnetic field application system.
07:04
Synthesis of Strong Adhesive Hydrogel, Gelatin O-Nitrosobenzaldehyde
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Cited by 1 •
2022
The protocol presented here shows the synthesis of a strong adhesive hydrogel gelatin o-nitrosobenzaldehyde (gelatin-NB). Gelatin-NB has rapid and efficient tissue adhesion ability, which can form a strong physical barrier to protect wound surfaces, so it is expected to be applied to the field of injury repair biotechnology.
08:35
Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
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Cited by 28 •
2012
Type B gelatin-based engineered nanovectors system (GENS) was developed for systemic gene delivery and transfection in the treatment of pancreatic cancer. By modification with epidermal growth factor receptor (EGFR) specific peptide on the surface of nanparticles, they could target on EGFR receptor and release plasmid under reducing environment, such as high intracellular glutathione concentrations.
06:29
Preparation of Metal-Organic Framework-Gelatin Hydrogels Through Coacervation
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2026
In this article, we present the preparation of MOF-gelatin hydrogels that benefit from the thermoreversible properties of gelatin, associated with its phase separation by coacervation. These hydrogel composites exhibit high MOF loading, large porosity, and a good spatial distribution of MOF particles within the gelatin matrix.
FAQs
The matrix keeps inoculated cells or microorganisms localized instead of allowing them to disperse throughout the nutrient medium. This spatial stability lets individual populations remain separated as they grow, making their development visible as distinct colonies. As a result, researchers can examine colony distribution, isolate populations, and compare growth patterns under the same culture conditions.
Cooling changes the nutrient medium from a molten state into a semisolid matrix that can retain nutrients and maintain inoculated material in place. This transition creates the stable surface needed for localized growth. The resulting physical structure therefore supports colony development and makes later observations of population separation and growth patterns possible.
The plate environment can be established with defined nutrients and other selected conditions, allowing researchers to observe how biological populations respond within a controlled setting. Differences in colony development may reveal phenotypic traits, contamination, or responses to environmental or chemical treatments. Consistent conditions also make comparisons among populations more interpretable.
Preparation begins by incorporating a gelling agent into a nutrient medium and using the molten mixture before it solidifies. After cooling produces the semisolid surface, biological samples are inoculated onto the plate. The localized populations can then develop into visible colonies, which are examined for isolation, growth patterns, phenotypes, or treatment responses.
Visible colonies provide a way to assess whether populations developed separately and to examine differences in their observable characteristics. These plates support colony isolation, phenotypic observation, and detection of contamination. They can also reveal growth patterns and changes associated with environmental or chemical treatments, linking culture appearance to biological responses.
They are useful when researchers need a stable, spatially defined culture environment for microbial cultivation or population comparison. Applications include isolating colonies, examining genetic traits through observable phenotypes, monitoring contamination, and studying responses to selected environmental or chemical treatments. Their solid format provides a practical basis for visually tracking biological growth.