Geobacter Shewanella

Geobacter and Shewanella are electroactive bacteria known for transferring electrons outside their cells, making them important models for microbial energy conversion, biogeochemical cycling, and environmental biotechnology. During anaerobic respiration, Geobacter commonly transfers electrons through c-type cytochromes and conductive pili to insoluble minerals or electrodes, whereas Shewanella can use outer-membrane cytochromes and secreted flavins as electron shuttles. These complementary strategies support microbial fuel cells, metal reduction, and contaminant transformation, while providing experimental systems for studying extracellular electron transfer, synthetic biology, and engineered bioelectrochemical technologies.

Geobacter Shewanella - Related Videos

Research

JoVE Journal - Chemistry

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

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Cited by 8 •

2017

This article presents a method for growing a biofilm for in situ time-of-flight secondary ion mass spectrometry for chemical mapping in its hydrated state, enabled by a microfluidic reactor, System for Analysis at the liquid Vacuum Interface. The Shewanella oneidensis MR-1 with green fluorescence protein was used as a model.

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

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Cited by 8 •

2018

Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.

Measuring the Kinetic Isotope Effect on Microbial Electron Transport Using Deuterium Oxide

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2025

Source: Tokunou, Y., et al, Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates a whole-cell electrochemical method to assess how proton transfer affects extracellular electron transport (EET) in electroactive bacteria. By replacing protons with deuterons using deuterium oxide, the method measures changes in current to reveal a kinetic isotope effect, highlighting the role of...

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