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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Biochemistry
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JoVE Journal Biochemistry
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

DOI:
10.3791/57584-v

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09:00 min

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April 16, 2018

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, ,
1Department of Applied Chemistry,University of Tokyo, 2Global Research Center for Environment and Energy based on Nanomaterials Science,National Institute for Materials Science

Chapters

  • 00:05Title
  • 01:00Formation of a Monolayer Biofilm of S. oneidensis MR-1 on an ITO Electrode
  • 04:11Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate
  • 05:24Addition of Deuterium Water to Measure the KIE on the EET Process
  • 06:26Results: Microbial Current Change Induced by D2O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.

Tags

Keywords: Deuterium Kinetic Isotope EffectExtracellular Electron TransportShewanella Oneidensis MR-1Electrochemical DetectionProton TransportC-type CytochromesThree-electrode Electrochemical ReactorITO SubstrateAnaerobic ConditionsLactateYeast Extract

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