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JoVE Journal
Biochemistry

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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
 

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Article DOI: 10.3791/57584 09:00 min April 16th, 2018
April 16th, 2018

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Summary

Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.

Transcript

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Electrochemical Detection Deuterium Kinetic Isotope Effect Extracellular Electron Transport Shewanella Oneidensis MR-1 Proton Transport Outer Membrane C-type Cytochromes Assay Native Proton Management KIE LB Medium Cell Suspension Defined Medium Lactate As Carbon Source Yeast Extract As Trace Elements
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