Intestinal Transcytosis
Intestinal Transcytosis - Related Videos
02:28
Assessing Transcytosis of E. coli Across Intestinal Epithelial Monolayers
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2025
Source: Islam, A., et al., Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates. J. Vis. Exp. (2023)This video demonstrates the procedure for assessing transcytosis of neonatal Escherichia coli across intestinal epithelial monolayers using transwell inserts, electrical resistance measurement, and bacterial quantification.
08:32
Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
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Cited by 1 •
2023
Escherichia coli causes sepsis in neonates who ingest the bacteria around the time of birth. The process involved in E. coli’s ability to travel from the enteric tract to the bloodstream is poorly understood. This in vitro model assesses the ability of E. coli strains to travel through the intestinal epithelial cells.
01:15
Transcytosis of IgG
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2023
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis. IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
00:58
Recycling Endosomes and Transcytosis
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2023
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route. The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
FAQs
The two membrane surfaces provide distinct entry and exit points for cargo. Endocytosis at the apical membrane brings material from the intestinal lumen into epithelial vesicles, while exocytosis at the basolateral membrane releases it toward underlying tissues and the circulation. This polarized arrangement allows epithelial cells to direct transported substances across the barrier rather than distributing them randomly within the cell.
Enterocytes and microfold, or M, cells support different biological functions within the intestinal epithelium. Enterocyte-associated transport contributes to absorption, including macromolecule uptake, whereas M-cell activity supports antigen sampling and the transfer of immune signals. Their participation connects epithelial transport with both acquisition of materials and communication between the gut lumen and immune processes.
Vesicles provide an organized intracellular route for cargo that is being transferred across an epithelial cell. Instead of requiring the substance to cross the cell cytoplasm directly, the cargo remains associated with transport vesicles as it moves from the lumen-facing surface to the tissue-facing surface. This mechanism is therefore relevant to the handling of macromolecules and other larger transported materials.
The pathway supports several biologically important cargo categories, including macromolecules, antigens, nutrients, immune signals, and materials considered for oral delivery. These functions are not identical: nutrient uptake concerns acquisition, antigen sampling concerns surveillance of luminal material, and immune-signal transfer concerns communication. Together, they show how one epithelial transport route can serve absorptive and immune-related roles.
In biology research, investigators can examine the pathway as a connection between epithelial absorption and immune surveillance. Nutrient-related studies focus on movement from the lumen toward underlying tissues and circulation, while mucosal-immunity studies emphasize antigen sampling and immune-signal transfer. Considering both roles helps researchers evaluate how intestinal epithelial cells interact with luminal contents and internal biological systems.
Its relevance comes from the ability of intestinal epithelial cells to move selected cargo from the gut lumen toward underlying tissues and the circulation. This makes the pathway a research focus for oral drug delivery, where investigators seek to understand how substances introduced through the digestive tract might cross the intestinal epithelial barrier. The same transport principle also informs studies of nanoparticle-based delivery.
Nanoparticle-based transport studies can investigate whether engineered particles interact with intestinal epithelial cells and whether they can be carried from the lumen toward underlying tissues or the circulation. The pathway provides a biological context for evaluating transport across the intestinal barrier, alongside research on nutrient uptake, antigen sampling, and oral delivery. These studies connect material transport with intestinal function and immune biology.