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Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
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Chapters
- 00:04Introduction
- 00:44Preparing Transcytosis Inserts with T84 Cells (Approximately 1 – 2 Weeks Before the Experiment)
- 03:43Preparing T84 Cells 1 Day Before the Experiment Using TCM w/o Antibiotics and Escherichia coli Cultures (Started 1 Day Before the Experiment)
- 04:19Preparing E. coli Inoculum, Epithelial Cells, and Materials (On the Morning of the Experiment)
- 05:05Inoculation of the Cells (Start of the Experiment)
- 05:54Quantifying the Transcytosis and End of the Experiment
- 06:56Results: Transcytosis of Neonatal E. coli Isolates
- 08:04Conclusion
Escherichia coli causes sepsis in neonates who ingest the bacteria around the time of birth. The process involved in E. coli’s ability to travel from the enteric tract to the bloodstream is poorly understood. This in vitro model assesses the ability of E. coli strains to travel through the intestinal epithelial cells.
